【Objective】 To compare the effects of two growth hormone(GH) provocative tests(arginine and levodopa combined or sequential ways) among children with short stature. 【Methods】 77 children with short stature were divided into 2 groups randomly. The combined group(39 cases) was given with arginine and levodopa at the same time to stimulate GH secretion, while the sequential group(38 cases) was given with arginine and levodopa separately in two days. The peak value of GH and the positive incidence of the two stimulation tests were compared. Peak value <10 ng/mL was defined as negative test and peak value >10 ng/mL was defined as positive test. 【Results】 The GH peak value in combined group and sequential group were(15.55±7.50) ng/mL and(13.80±9.23) ng/mL respectively. The incidence of positive test in combined group(82.0%) was significantly higher than sequential group(60.5%). 【Conclusion】 The GH provocative test performed in combined way has a higher positive incidence than in sequential way, and is more reliable in diagnosing GH deficiency.