Abstract: Objective To discuss the differences between Ningxia Hui and Han asthmatic children about the single nucleotide polymorphisms of locus of BsmI,ApaI,TaqI and FokI on the VDR gene. Methods The peripheral blood DNA from Hui and Han asthmatic children was extracted to analyze the genotype and gene frequency of dynamic locus of VDR gene using the methods of PCR and PCR-RFLP,Chi square test was used to analyse data. Results FokI was a locus where the difference of asthma existed between Ningxia Hui and Han asthmatic children(P<0.05).There was no distinguished difference about the distribution of gene frequency and genotype frequency of the locus of BsmI,ApaI and TaqI existed between the Hui asthma group and Han asthma group(P>0.05). Conclusions Among the four polymorphic sites being studied,the following results have been achieved:FokI is a locus where the differences exist between Ningxia Hui children's asthma and Ningxia Han children's asthma.There is no difference between Ningxia Hui and Han children's asthma about the locus of BsmI,ApaI and TaqI.

Key words: bronchial asthma, VDR gene, single nucleotide polymorphism, children

摘要： 目的 探讨VDR基因BsmI、ApaI、TaqI和FokI位点的单核苷酸多态性在宁夏回汉族哮喘儿童之间的差异性。 方法 提取回汉族哮喘儿童外周血DNA各60例,采用PCR及PCR-RFLP方法分析VDR基因四个多态位点的基因型及基因频率,采用χ²检验分析相关数据。 结果 FokI位点是宁夏回汉族儿童哮喘之间存在差异性的一个位点(P<0.05)。BsmI、ApaI及TaqI位点在宁夏回汉族儿童哮喘之间差异无统计学意义(P>0.05)。 结论 在所研究的VDR基因的四个多态位点中:FokI位点在宁夏回汉族儿童哮喘之间存在差异性;其余位点与在宁夏回汉族儿童之间差异无统计学意义。

关键词: 支气管哮喘, VDR基因, 单核昔酸多态, 儿童