Objective To discuss the differences between Ningxia Hui and Han asthmatic children about the single nucleotide polymorphisms of locus of BsmI,ApaI,TaqI and FokI on the VDR gene. Methods The peripheral blood DNA from Hui and Han asthmatic children was extracted to analyze the genotype and gene frequency of dynamic locus of VDR gene using the methods of PCR and PCR-RFLP,Chi square test was used to analyse data. Results FokI was a locus where the difference of asthma existed between Ningxia Hui and Han asthmatic children(P<0.05).There was no distinguished difference about the distribution of gene frequency and genotype frequency of the locus of BsmI,ApaI and TaqI existed between the Hui asthma group and Han asthma group(P>0.05). Conclusions Among the four polymorphic sites being studied,the following results have been achieved:FokI is a locus where the differences exist between Ningxia Hui children's asthma and Ningxia Han children's asthma.There is no difference between Ningxia Hui and Han children's asthma about the locus of BsmI,ApaI and TaqI.
Key words
bronchial asthma /
VDR gene /
single nucleotide polymorphism /
children
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] 田薇,陈艳,赵江霞,等.维、哈及汉族儿童维生素D受体FOKI位点多态性研究[J].新疆医科大学学报,2008,31(4):430-432.
[2] 中华医学会儿科学会呼吸学组.儿童支气管哮喘诊断与防治指南[J].中华儿科杂志,2008,46(10):745-753.
[3] Raby BA,Silverman EK,Lazarus R,et al.Chromosome 12q harbors multiple genetic loci related to asthma and asthma-related phenotypes[J].Hum Mol Genet,2003,12(16):1973-1979.
[4] 刘瑾,张文昌.1,25-二羟维生素D3与维生素D受体的研究.海峡预防医学杂志,2007,13(2):29-31.
[5] D'Ambrosio D,Cippitelli M,Cocciolo MG,et al.Inhibition of IL-12 production by 1,25dihydroxyvitamin D3:involvement of NF-B downregulation in transcriptional repression of the p40 gene[J].J Clin Invest,1998,101(1):252-262.
[6] Reichel H,Koeffler HP,Tobler A,et al.1,25-Dihydroxyvitamin D3 inhibits-interferon synthesis by normal human peripheral blood lymphocytes[J].Proc Natl Acad Sci USA,1987,84(10):3385-3389.
[7] Lemire JM,Archer DC,Beck L,et al.Immunosuppressive actions of 1,25dihydroxyvitamin D3:preferential inhibition of Th1 functions[J].J Nutr,1995,125(6S):1704-1708.
[8] Saadi A,Gao G.Association study between vitamin D receptor gene polymorphisms and asthma in the Chinese Han population:a case-control study[J].BMC Med Genet,2009,10:7180.
[9] Poon AH,Laprise C,Lemire M,et al.Association of Vitamin D receptor genetic Variants with susceptibility to asthma and atopy[J].AM J Rspir Crit Care Med,2004,170(9):967-973. [10] Raby BA,Lazarus R,Silverman EK,et al.Association of vitamin D receptor gene polymorphisms with childhood and adult asthma[J].Am J Respir Crit Care Med,2004,170(10):1057-1065.
PDF(557 KB)
