Abstract: Objective To explore the effect of perinatal high energy on lipid metabolism in SD rat offspring. Methods A total of 15 pregnant SD rats were divided randomly into control group,high fat group and high fat and high carbohydrate group,which were fed with lab chow,high fat diet(containing 20% of lard) and high fat and high carbohydrate diet(containing 20% of lard and 20% of sucrose) respectively.The pups of 4 weeks old were killed,and their body weight,the mass of liver and visceral fat and the levels of plasma TC,TG,HDL and leptin were determined. Results With aspects of body weight,the mass of liver and visceral fat,the data of high fat group(90.05±6.71),(3.73±0.18) and (0.41±0.12)g and high fat and high carbohydrate(89.82±5.08),(3.82±0.27) and (0.41±0.13)g were higher than those of control group(70.60±17.30),(2.87±0.83) and (0.22±0.07)g (P＜0.05);The level of plasma TC of high fat and high carbohydrate(1.72±0.10) was higher than that of high fat group(0.9±0.33) and control group(0.8±0.3)(P＜0.05);The levels of plasma TG of high fat group (0.83±0.54) and high fat and high carbohydrate group(1.02±0.22) were higer than that of control group(0.45±0.21)(P＜0.05);The level of plasma HDL and leptin of high fat group(0.23±0.08),(1.16±0.06) and high fat and high carbohydrate group(0.26±0.09),(1.15±0.04) were higher than those of control group(0.50±0.03),(1.26±0.08)(P＜0.05).Conclusion Perinatal high energy leads lipid dysfunction including obesity,disorder of plasma lipid,decline of plasma leptin and so on.

Key words: lipid metabolism, high energy diet, SD rat, leptin

摘要： 目的 探索SD大鼠孕哺期给予高能量饲料对子代脂代谢的影响。 方法 成年SD孕鼠15只随机分为对照组、高脂组、高脂高糖组,在孕哺期分别给予标准鼠粮、高脂饲料(含20%猪油)和高脂高糖饲料(含20%猪油和10%蔗糖)。仔鼠4周处死,称量体重、肝脏和肾周脂肪质量,并检测血浆甘油三酯、胆固醇、高密度脂蛋白和瘦素水平。结果 体重、肝脏和肾周脂肪质量,高脂组[(90.05±6.71)、(3.73±0.18)和(0.41±0.12)g]和高脂高糖组[(89.82±5.08)、(3.82±0.27)和(0.41±0.13)g]均高于对照组[(70.60±17.30)、(2.87±0.83)和(0.22±0.07)g](P＜0.05);高脂高糖组总胆固醇水平(1.72±0.10)高于高脂组(0.9±0.33)和对照组(0.8±0.3)(P＜0.05);高脂组(0.83±0.54)和高脂高糖组(1.02±0.22)的甘油三脂水平高于对照组(0.45±0.21)(P＜0.05);高密度脂蛋白和瘦素,高脂组(0.23±0.08,1.16±0.06)和高脂高糖组(0.26±0.09,1.15±0.04)的低于对照组(0.50±0.03,1.26±0.08)(P＜0.05)。 结论SD大鼠孕哺期摄入高能量饲料造成子代肥胖、血脂异常和血浆瘦素水平下降等脂代谢紊乱。

关键词: 脂代谢, 高能量饲料, SD大鼠, 瘦素