Abstract: Objective To observe the influence of extrinsic estrogen on the reproductive system of prepubertal male rats. Methods A total of 45 rats were randomly divided into high dose group,low dose group and control group.E2B was administered through gastric tube in male rats with 15 000 μg/kg,15 μg/kg,0 μg/kg,dose respectively.One time every two days,after three weeks,normal breeding to ninth weeks,then put to death and draw materials,and measured corresponding index. Results Compared with control group,T concentration of low dose decreased(P<0.05),but FSH,LH and E2 concentration increased,the differences were statistically significant (P<0.01).Four kinds of hormone of high dose changed more apparent(P<0.01).In two experimental group,the differences of T,FSH,LH and E2 concentration had statistical significances (P<0.05).The testicular reproductive small tube and AGD of two experimental group were both abnormal changed,but changes in high dose group were more obvious than those in low group.Conclusion EES can change the normal serum sex hormone levels and reproductive small tube of preadolescence male rats.

Key words: extrinsic estrogen, estradiol benzoate, preadolescence, rats, reproductive system

摘要： 目的 观察外源性雌激素对青春期前雄性大鼠生殖系统的影响。 方法 将45只大鼠随机分为用高、低剂量组和对照组,苯甲酸雌二醇(E2B)染毒剂量分别为15 000 μg/kg、15 μg/kg,0 μg/kg灌胃摄入,对照组给予同等体积玉米油,隔日1次,在分别作用3个周后正常饲养至生后9周,处死取材,测定相应指标。结果 与对照组相比,低剂量组睾酮(testosterone,T)浓度降低(P<0.05),卵泡刺激素(follicle-stimulating hormone,FSH)、黄体生成素(luteinizing hormone,LH)及雌二醇(estrodiol,E2)浓度升高,差异均有统计学意义(P<0.01)。高剂量组4种激素水平改变更显著(P<0.01)。另外,两实验组相比,T、FSH、LH及E2浓度的差异也有统计学意义(P<0.05)。两实验组睾丸生殖小管、肛生殖距(AGD)均有异常改变,高剂量组改变较低剂量组明显。 结论外源性雌激素可改变青春期前雄性大鼠血清性激素水平,并使生殖小管的正常结构和形态发生异常改变。

关键词: 外源性雌激素, 苯甲酸雌二醇, 青春期前, 大鼠, 生殖系统