Abstract: 【Objective】 To study the pathogenic characterization of the hand-foot-mouth diseases (HFMD) in Cixi in 2011,and to understood the HFMD epidemic trend. 【Method】 Sentinel hospital surveillance for clinical diagnosis of HFMD case specimens were collected.Real-time fluorescence(Real-time) RT-PCR assay was used to detect human enteric viruses (HEV),enterovirus 71 (EV71)and Coxsackie virus group A type 16 (CVA16) of specific nucleic acid. 【Results】 Positive rate of total intestinal virus specimen was 89.97% in 289 cases; Enterovirus 71 and Coxsackie A16 were 58.46% and 22.31% respectively,other type accounted for 19.23%.High positive rate was found in 1~5 years old and male children patients;EV71 positive rate of hospitalized cases was 68.97%. 【Conclusion】 EV71 and CVA16 are the major pathogens of HFMD in Cixi,EV71 infection is much likely to cause severe cases and inpatients,in some area CVA16 is the dominant species.

Key words: hand-foot-mouth disease, etiology, EV71, CVA16, children

摘要： 【目的】 研究2011年慈溪市儿童手足口病(hand-foot-mouth disease,HFMD)病原学特征,了解本地区HFMD流行趋势。 【方法】 收集监测哨点医院上送的临床诊断为HFMD病例标本,应用实时荧光(Real-time)RT-PCR法检测标本中的人肠道病毒(HEV)、肠道病毒71型(EV71)和柯萨奇病毒A组16型(CVA16)特异性核酸。 【结果】 289例标本中检出总肠道病毒阳性病例260例(89.97%),其中EV71占总阳性率的58.46%,CVA16占总阳性率的22.31%,肠道其他型19.23%;病例年龄集中在1~5岁;男女性阳性比例为1.49∶1;住院病例EV71阳性率为68.97%。 【结论】 EV71和CVA16为本地手足口病的主要病原体,EV71是引起重症和住院病例的优势毒株型,在局部区域内CVA16型占主导。

关键词: 手足口病, 病原学, 肠道病毒71型, 柯萨奇A16型, 儿童