【Objective】 To investigate the drug-resistance of staphylococcus aureus(SAU) isolated from children under 5-year in Zhuhai area. 【Methods】 245 stains of SAU were isolated from 4 357 cases under 5-year in the Zhuhai Municipal Maternity and Child Health Care Hospital since January 2007 to December 2009. Kurby-Bauer method was used to analyze antimicrobial susceptibility of above-mentioned strains against 12 antimicrobial agents. Results were evaluated according to Clinical and Laboratory Standards Institute(CLSI) of that year. The data was statistic analysis by WHONET 5.4 and SPSS 17.0. 【Results】 95.1% of the cases were under 1-year. 97.1% strains were isolated from the specimens of the outpatients and in-patients within 48 h of hospitalization. The majority of the specimens was sputum(89%) and pneumonia was the main disease among the cases(88.6%). The prevalence of the methicillin-resistant staphylococcus aureus(MRSA) was 17.6%. The rates of resistance to penicillin, clindamycin and erythromycin were 93.9%, 51.4%, 67.6% respectively. All were sensitive to vancomycin and linezolid. The drug-resistance of MRSA was higher than methicillin sensitive staphylococcus cureus(MSSA)(P<0.05). According to the drug resistance of SAU, there was no significant difference between the newborns and the infants(P>0.05). 【Conclusions】 The detection rate of MRSA is low in children under 5-year with SAU infection in Zhuhai area. The drug-resistance rate is not high in the microbial agents except Penicillin, Clindamycin and Erythromycin. Oxacillin is still a cheap and sensitive drug to cure the children with SAU infection in Zhuhai area.