Objective To provide reference for the clinical diagnosis and analysis of neonatal seizure. Methods 60 cases of neonatal seizure were totally summarized and the general states,clinical manifestations,modes on set and video electroencephalograms were analyzed. Results The most common causes of neonatal seizure in 60 cases were neonatal hypoxic ischemic encephalopathy(33.3%),infection(13.3%),hypoglycemia(10.0%),intracranial hemorrhage(8.3%) and electrolyte disturbances(8.3%).The most common mode of onset were minimal seizure(41.7%),then clonic seizure(29.1%).The results of 28 (46.7%)cases of video electroencephalogram were normal,and 32 were abnormal,about 53.3%. Conclusions Neonatal seizure are caused by many types of disease.In clinical work,careful observation and necessary examinations,such as video electroencephalogram,are useful to diagnosis and pathogenesis.So better improvements and decrease of sequela of nervous system could be obtained.