足月新生儿呼吸窘迫综合征SP-B+1580基因突变分析

中国儿童保健杂志 ›› 2013, Vol. 21 ›› Issue (7): 682-684.

足月新生儿呼吸窘迫综合征SP-B+1580基因突变分析

尹晓娟,韩笑,范含笑,谢露,曲雯雯,封志纯

北京军区总医院附属八一儿童医院,北京 100700

收稿日期:2012-12-12 发布日期:2013-07-06 出版日期:2013-07-06
通讯作者: 封志纯,E-mail:drfengzc@fmmu.edu.cn
作者简介:尹晓娟(1966-),女,湖南人,硕士研究生导师,主要从事新生儿疾病的基础与临床研究。
基金资助:
国家自然科学基金资助项目(30871397)

Analysis of gene mutation in surfactant protein B +1580 site on full-term newborns with respiratory distress syndrome.

YIN Xiao-juan,HAN Xiao,FAN Han-xiao,XIE Lu,QU Wen-wen,FENG Zhi-chun.

Affiliated Bayi Children's Hospital,Beijing Military Region General Hospital,Beijing 100700,China

Received:2012-12-12 Online:2013-07-06 Published:2013-07-06

摘要/Abstract

摘要： 目的分析SP-B+1580基因突变与足月新生儿呼吸窘迫综合征(respiratory distress syndrome,RDS)的相关因素。方法 312例汉族足月RDS新生儿作为RDS组和156例汉族足月健康新生儿作为对照组。PCR扩增 SP-B exon4并测序;免疫组化技术分析肺部SP-B 和proSP-B表达。结果两组 SP-B+1580 位点存在CC、C/T、TT 3种基因型,RDS组C/T基因型频率明显高于对照组(P<0.05)。具有C/T基因型的RDS患儿,剖宫产、机械通气、死亡的发生率较其他基因型高,胸片显示RDS程度较重、需要肺表面活性物质的剂量大[200 mg/(kg·次),3~4剂](P<0.01)。50例伴有C/T基因型的RDS死亡患儿中有19例患儿成熟SP-B和proSP-B蛋白肺部表达减少。结论 SP-B+1580位点C/T基因型可引起成熟SP-B和proSP-B蛋白在肺部的减少,是足月新生儿RDS发病的高危因素。

关键词: 呼吸窘迫综合征, 足月, 新生儿, 表面活性物质蛋白B, 基因突变

Abstract: Objective To analyze correlative factor between gene mutation (SP-B +1580) and respiratory distress syndrome(RDS) in full-term newborns. Methods There were 312 Han full-term newborns with RDS as RDS group and 156 Han healthy full-term newborns as control group.Genotypic analysis on exon 4 was performed with polymerase chain reaction amplification(PCR) and gene sequencing.Analysis of for expression of SP-B and proSP-B in lung were determined with immunohistochemical technique. Results Three genotypes of SP-B+1580 including CC,C/T,TT were discovered in two groups.Frequency of C/T genotype in RDS group was higher than that in control group(P<0.05).Patients with C/T genotype had higher rates on uterine-incision delivery mechanical ventilation and mortality chest X-ray grade showed severity about RDS,and needed more pulmonary surfactants(P<0.01).19 patients with decreased mature SP-B and proSP-B were among 50 cases of dead patients with C/T genotypes. Conclusion C/T genotype in SP-B +1580 site causing decreased mature SP-B and proSP-B may be the high risk for RDS.

Key words: respiratory distress syndrome, full-term, newborn, surfactant protein B, gene mutation

中图分类号:

R725.6

尹晓娟,韩笑,范含笑,谢露,曲雯雯,封志纯. 足月新生儿呼吸窘迫综合征SP-B+1580基因突变分析[J]. 中国儿童保健杂志, 2013, 21(7): 682-684.

YIN Xiao-juan,HAN Xiao,FAN Han-xiao,XIE Lu,QU Wen-wen,FENG Zhi-chun.. Analysis of gene mutation in surfactant protein B +1580 site on full-term newborns with respiratory distress syndrome.[J]. journal1, 2013, 21(7): 682-684.

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