Diversity analysis of predominant intestinal microflora in children with Cow's milk protein allergy.

BAI Hong-jian; GUO Ling; HAN Ying; LI Ai-li; DU Peng; YUAN Xiu-juan; JIANG Yan.

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Chinese Journal of Child Health Care ›› 2014, Vol. 22 ›› Issue (1) : 10-13.

Diversity analysis of predominant intestinal microflora in children with Cow's milk protein allergy.

BAI Hong-jian¹, GUO Ling¹, HAN Ying², LI Ai-li¹, DU Peng¹, YUAN Xiu-juan¹, JIANG Yan¹.

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Abstract

Objective To investigate the diversity of predominant intestinal microflora in children with Cow's milk protein allergy. Methods Collecting the feces from 7 initially diagnosed cases of children with Cow's milk protein allergy and 5 healthy control participants.The universal primer of the bacterial 16S rDNA-V3 region was used as primer and the total genomic bacterial DNA were used as a template.After PCR amplification, denaturing gradient gel electrophoresis (DGGE) analysis was performed.The Results analysis was applied by Quantity One software. Results There was significant difference in the diversity of the predominant intestinal microflora in children with Cow's milk protein allergy and healthy control, and the intestinal microflora diversity of Cow's milk protein allergy was higher.The participants in allergy group and control group could be divided into different two clusters.The Results indicated that the predominant intestinal microflora in the two groups were different.Similarity analysis showed that allergic group similarity was range from 27%~72%; the healthy control group similarity was range from 28%~64%. Conclusions The diversity of the predominant intestinal microflora in children with Cow's milk protein allergy changed significantly compared with healthy children.Intestinal microecological balance might be the underlying causes of cow's milk protein allergy.

Key words

Cow's milk protein allergy / PCR- Denaturing Gradient Gel Electrophoresis / intestinal microflora / children

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BAI Hong-jian, GUO Ling, HAN Ying, LI Ai-li, DU Peng, YUAN Xiu-juan, JIANG Yan.. Diversity analysis of predominant intestinal microflora in children with Cow's milk protein allergy.[J]. Chinese Journal of Child Health Care. 2014, 22(1): 10-13

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