Objective To study on etiology of diarrhea among children and to provide the scientific basis for the prevention of the virus diarrhea disease. Method Using epidemiological method to survey patients with diarrhea,virus was detected by enzyme linked immuno assy (ELISA) and revere-transcription polymeras chain reaction (RT-PCR ). Results Virological testing results showed:the rate positive of virus was 53.37%(222/416),40.87% of Rotavirus(170/416),6.97% of human caliciviruses (29/416),0.96% of Astrovirus (4/416),4.57% of Adenovirus (19/416).8.17% of Mixed infection (34/416).The distribution of season:HRV infected highest was December and follow year of January.HuCV infected highest was November and December.AdeV infected highest was December and June.Astv infected highest was January and February.The distribution of month -old of Virus diarrhea:HRV,HuCV,AdeV,AstV of infection most was 12~ month groups (45.23%,38.29%,43.75%,29.41%).66.67% of HRVG3 and p[8](68.13%),p[4](20.88%) were most common stain.23.58% of HRV G9 serotype rose significantly. Conclusions HRV is mainly pathogen of virus diarrhea in children,follow HuCV,AdeV,Astv.G3 and P[8],P[4] of the HRV are epdmics trains,the follow is G9.These results provide important information for HRV vaccine research.
Key words
diarrhea /
virus /
styping /
HRVG9
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