【Objective】 To investigate the protective effects of hypothermia to the grey matter and the periventricular white matter of the brains of the neonatal rats with hypoxic-ischemic brain damage(HIBD). 【Methods】 The 7d neonatal rats got the HIBD model. Hypothermias of 31 ℃, 34 ℃ were given to them for 3 h immediately after the operation. The degrees of grey of apoptosis, p16, bcl-2 of the cerebral cortex, the hippocampi tissue and the periventricular white matter in the brains of rats was used to observe when 24, 72, 168 h after the operation. 【Results】 1)The degrees of grey of p16: It was higher in the HIBD group than that in the pseuoperation group, the peak was at 24 hours after HI, it was deduced in 31 ℃ group, it was not deduced in 34 ℃group,but the peak was delated . 2) The degrees of grey of apoptosis: It was the same as that of p16. 3) The degrees of grey of bcl-2: It was the same as that of p16,it were deduced 24, 72 hours after HI in the 34 ℃ hypothermia group . 4) Correlation: there were the correlation ships (P<0.01) between either two of the degrees of the greys of p16, apoptosis, bcl-2 in all the brain tissues. 【Conclusions】 Hypothermia intervention could obviously deduce the express of p16, bcl-2, deduce apoptosis or delate their peaks of neonatal rats, brains with HIBD, so protected their grey matter tissue and the white matter tissue.
Key words
hypothermia /
ischemic brain damage /
white matter damage /
apoptosis
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