Relationship between the apoptosis of mononuclear cell and the expression of cytokines and insulin-like growth factor-1 in patients with henoch-schonlein purpura
- CHENG Han-rong, WEN Fei-qiu, WU Ben-qing
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Abstract
【Objective】 To observe the changes of mononuclear cell (MNCs) apoptosis and the level of inflammatory cytokines and IGF-1 in patients with henoch-schonlein purpura (HSP), and to study the relationship between them. 【Methods】 The apoptosis of MNCs in 36 patients with HSP and 22 healthy controls were determined with flow cytometry. The inflammatory cytokines and theIGF-1 were tested with ELISA method. 【Results】 The percentage of MNCs apoptosis in the patients with HSP was significantly higher than that of healthy controls. The levels of IL-8, TNF-α, IL-6, IGF-1 in patients with HSP were significantly higher than those of healthy controls, and the increased levels of inflammatory cytokines and IGF-1 in HSP patients were positively correlated to the percentage of MNCs apoptosis. 【Conclusions】 The data demonstrates that there is a promote apoptotic process of MNCs in active HSP. The high expression of inflammatory cytokines and IGF-1 may play an important role of promoting MNCs apoptosis which reduces the functional life span of MNCs. For this reason, modulating the expression of cytokines, IGF-1 and moderately controlling the apoptosis of MNCs may be a new therapeutic target for HSP treatment.
Key words
henoch-schonlein purpura / apoptosis / cytokines / mononuclear cell
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References
[1] Riganta D. Clinical overview of vasculitic syndromes in the pediatric age [J]. Eur-Rev-Med-Pharmacol-Sci,2006,10(6):337-345.
[2] Ozaltin F, Besbas N, Uckan D, et al. The role of apoptosis in childhood henoch-schonlein purpura[J]. Clinical rheumatology,2003,22(4/5):265-267.
[3] Carlo-del VG, Rosa P, Maria A, et al. Cytokine pattern and endothelium damage markers in Henoch-Schonlein purpura.[J]. Immunopharmacology and Immunotoxicology, 2008, 30(3):623-629.
[4] 胡亚美,江载芳.诸福棠实用儿科学(上册)[M].7版. 北京:人民卫生出版社,2005:688-690.
[5] Prais D, Amir J, Nussinovitch M. Recurrent henoch-schonlein purpura in children [J]. J Clin Rheumatol, 2007,13(10):25-28.
[6] Yang YH, Wang SJ, Chuang YH, et al. The level of IgA antibodies to human umbilical vein endothelial cells canbe enhancedby TNF-alpha treatment in children with Henoch-Schonlein purpura[J]. Clin Exp Immunol,2002,130:352-357.
[7] Yu YH, Pan KL, Li Q, et al. Roles of serum and urinary interleukins 13Ralpha2 and other cytokines in pediatricHenoch-Schonlein purpura[J]. Zhongguo Dang Dai Er Ke Za Zhi,2009,11(1):37-40.
[8] Duan B, Morel L. Role of B-1a cells in autoimmunity[J]. Autoimmun Rev,2006,5:403-408.
[9] Yang YH, Huang YH, Lin YL, et al. Circulating IgA from acute stage of childhood Henoch-Schonlein purpura can enhance endothelial interleukin (IL)-8 production through MEK/ERK signalling pathway[J]. Clin Exp Immunol,2006,144:247-253.
[10] Yildiz B, Kural N, Aydin BM, et al. Increased serum levels of insulin-like growth factor (IGF)-1 and IGF-binding protein-3 in Henoch-Schonlein purpura[J]. The Tohoku Journal of Experimental Medicine,2008,214(4):333-340.
[2] Ozaltin F, Besbas N, Uckan D, et al. The role of apoptosis in childhood henoch-schonlein purpura[J]. Clinical rheumatology,2003,22(4/5):265-267.
[3] Carlo-del VG, Rosa P, Maria A, et al. Cytokine pattern and endothelium damage markers in Henoch-Schonlein purpura.[J]. Immunopharmacology and Immunotoxicology, 2008, 30(3):623-629.
[4] 胡亚美,江载芳.诸福棠实用儿科学(上册)[M].7版. 北京:人民卫生出版社,2005:688-690.
[5] Prais D, Amir J, Nussinovitch M. Recurrent henoch-schonlein purpura in children [J]. J Clin Rheumatol, 2007,13(10):25-28.
[6] Yang YH, Wang SJ, Chuang YH, et al. The level of IgA antibodies to human umbilical vein endothelial cells canbe enhancedby TNF-alpha treatment in children with Henoch-Schonlein purpura[J]. Clin Exp Immunol,2002,130:352-357.
[7] Yu YH, Pan KL, Li Q, et al. Roles of serum and urinary interleukins 13Ralpha2 and other cytokines in pediatricHenoch-Schonlein purpura[J]. Zhongguo Dang Dai Er Ke Za Zhi,2009,11(1):37-40.
[8] Duan B, Morel L. Role of B-1a cells in autoimmunity[J]. Autoimmun Rev,2006,5:403-408.
[9] Yang YH, Huang YH, Lin YL, et al. Circulating IgA from acute stage of childhood Henoch-Schonlein purpura can enhance endothelial interleukin (IL)-8 production through MEK/ERK signalling pathway[J]. Clin Exp Immunol,2006,144:247-253.
[10] Yildiz B, Kural N, Aydin BM, et al. Increased serum levels of insulin-like growth factor (IGF)-1 and IGF-binding protein-3 in Henoch-Schonlein purpura[J]. The Tohoku Journal of Experimental Medicine,2008,214(4):333-340.
