Research on the effect on the incidence of the fetal rats with fetal growth restriction and the apoptosis of placental cells with maternal multiple micronutrients supplementation.

ZONG Lu; GOU Wen-li; HUANG Pu; XUE Qian; LV Ye.

doi:10.11852/zgetbjzz2016-24-04-10

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Chinese Journal of Child Health Care ›› 2016, Vol. 24 ›› Issue (4) : 373-376. DOI: 10.11852/zgetbjzz2016-24-04-10

Research on the effect on the incidence of the fetal rats with fetal growth restriction and the apoptosis of placental cells with maternal multiple micronutrients supplementation.

ZONG Lu¹,GOU Wen-li¹,HUANG Pu¹,XUE Qian²,LV Ye³.

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Abstract

Objective To explore the effect on the incidence of the fetal rats with fetal growth restriction(FGR) and the apoptosis of placental cells with maternal multiple micronutrients (MMN) supplementation. Methods FGR model of SD rats was done by maternal passive smoking.The pregnant rats were divided into four groups (FGR,MMN,control and normal group).Rats of the MMN group and the control group were fed with supplementation of MMN,and the other rats of the FGR group and the normal group were fed on normal food.Rats of the FGR group and the MMN group endured passive smoking,and other rats of the control group and the normal group breathed normally.The number and the weight of the fetal rats,the weight of the placentas were contrasted.Cell apoptosis of the placentas were detected by method TUNNEL. Results The number,the weight of fetal rats and placenta of the FGR group decreased significantly than those of control group (P<0.05).The number and the weight of the fetal rats of the MMN group increased significantly than those of FGR group(P<0.05),but there was no signicant difference between the placental weight of these two groups(P>0.05).There existed placental cellular apoptosis in all four groups.The apoptosis index of FGR group was higher than that of normal group(P<0.05); the apoptosis index of MMN group was lower than that of FGR group(P<0.05),with no difference with the normal group (P>0.05).All data of the control group had no signicant differences between the control group and the normal group(P>0.05). Conclusions Maternal MMN supplementation could decrease the incidence of FGR induced by passive smoking.The probable mechanism may be restrain abnormal placental cell apoptosis.

Key words

fetal growth restriction / placental / apoptosis / multiple / micronutrient

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ZONG Lu,GOU Wen-li,HUANG Pu,XUE Qian,LV Ye.. Research on the effect on the incidence of the fetal rats with fetal growth restriction and the apoptosis of placental cells with maternal multiple micronutrients supplementation.[J]. Chinese Journal of Child Health Care. 2016, 24(4): 373-376 https://doi.org/10.11852/zgetbjzz2016-24-04-10

References

[1] Erwin VV,Elisenda E,Miriam I,et al.Metabolomics reveals metabolic alterations by intrauterine growth restriction in the fetal rabbit brain[J].PloS One,2013,8(5):e64545-549.
[2] Thomas J,Theresa LP.Role of placental nutrient sensing in developmental programming[J].Clin Obstet Gynecol,2013,56(3):591-601.
[3] Longtine MS,Nelaon DM.Placental dysfunction and fetal programming:the importance of placental size,shape,histopathology,and molecular composition[J].Semin Reprod Med,2011,29(3):187-196.
[4] Song Z,Timothy RHR,Paige LB,et al.Placental adaptations in growth restriction[J].Nutrients,2015,7(1):360-389.
[5] 尉建霞,于松.胎盘相关因素与胎儿生长受限的研究进展[J].中国现代医生,2015,53(16):157-160.
[6] 宗璐,郭玉琳,苟文丽,等.孕期补锌对胎儿生长受限仔鼠学习记忆能力改变的研究[J].中国儿童保健杂志,2015,23(10):5-9.
[7] 陈竺,黄洁夫,刘谦,等.中国吸烟危害健康报告[R].北京:中华人民共和国卫生部,2012:5-40.
[8] 王畅,王声湧,董晓梅.孕妇被动吸烟对妊娠结局及后代健康的危害[J].中华疾病控制杂志,2010,14(11):1142-1145.
[9] Melissa AS,Amber MA,Kjersti MA.Maternal smoking as a model for environmental epigenetic changes affecting birthweight and fetal programming[J].Mol Hum Reprod,2013,19(1):1-6.
[10] Hung TH,Chen SF,Liou JD,et al.Bax,bak and mitochondrial oxidants are involved in hypoxia-reoxygenation-induced apoptosis in human placenta[J].Placenta,2008,29(7):565-583.
[11] Vedang AL,Tracy LN,Abhik D,et al.Vitamin A supplementation in extremely low-birth-weight infants:subgroup analysis in small-for-gestational-age infant[J].Am J Perinatol,2013,30(9):771-780.
[12] Noelle M,Daniel B.The fetal oringins of the metabolic syndrome:can we intervene?[J].J Pregnancy,2012,28(6):482690-482699.
[13] Christopher D,Krishnamachari S,Tinku T,et al.Vitamin B12 supplementation during pregnancy and early lactation increases maternal,breast milk,and infant measure of vitamin B-12 status[J].J Nutr,2014,144(5):758-764.
[14] Lee BE,Hong YC,Lee KH,et al.Influence of maternal serum levels of vitamins C and E during the second trimester on birth weight and length[J].Eur J Clin Nutr,2004,58(10):1365-1371.
[15] Tahmasebi B,Naqhdi N,Shahbazi M,et al.The effect of severe zinc deficiency and zinc supplement on spatial learning and memory[J].Biol Trace Emem Res,2009,130(1):48-61.
[16] Fawzi WW,Msamanga GI,Spiegelman D,et al.Randomised trial of effects of vitamin supplements on pregnancy outcomes and T cell counts in HIV-1-infected women in Tanzania[J].The Lancet,1998,351(9114):1477-1482.
[17] Osrin D,Vaidya A,Shrestha Y,et al.Effects of antenatal multiple micronutrient supplementation on birthweight and gestational duration in Nepal:double-blind,randomised controlled trial[J].The Lancet,2005,365(9463):955-962.
[18] Fenech M,Ferquson LR.Vitamins/minerals and genomic stability in humans[J].Mutat Res,2001,475(1-2):1-6.
[19] Williams PJ,Bulmer JN,Innes BA,et al.Possible roles for folic acid in the regulation of trophoblast invasion and placental development in normal early human pregnancy[J].Biol Reprod,2011,84(6):1148-1153.
[20] Fu QL,Wen X,Cui PG,et al.Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes[J].Int J Mol Med,2012,29(4):593-600.