Expression of optic atrophy 1 in renal tubular epithelial cells with hypoxia/re-oxygenation and the interventional effects of rhEPO

LIAO Kai-nan; ZOU Li-le; DONG Wen-bin

doi:10.11852/zgetbjzz2016-24-02-07

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Chinese Journal of Child Health Care ›› 2016, Vol. 24 ›› Issue (2) : 134-136. DOI: 10.11852/zgetbjzz2016-24-02-07

Expression of optic atrophy 1 in renal tubular epithelial cells with hypoxia/re-oxygenation and the interventional effects of rhEPO

LIAO Kai-nan¹,ZOU Li-le²,DONG Wen-bin³

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Abstract

Objective To investigate the expression of optic atrophy 1 (OPA1) in renal tubular epithelial cells with hypoxia/re-oxygenation (H/R) and to evaluate the effects of recombinant human erythropoietin (rhEPO) on it. Methods The cultured human kedney proxiamal tubular cell-2 (HK-2) cells in vitro were randomly divided into the following groups:normal control group,H/R group and rhEPO group.The expression of OPA1 protein was detected by immunohistochemistry,the rate of apoptosis cells was detected by flow cytometry. Results Compared with control group,the change in morphology of HK-2 was most serious and obvious,and the apoptosis rate of cells was obviously increased and the expression of OPA1 was significantly decreased in H/R group.But compared with H/R group,the expression of OPA1 were significantly increased in rhEPO group and the apoptosis rate of cells were decreased. Conclusion RhEPO has protective effect on renal tubular epithelial cell injury induced by hypoxia/re-oxygenation,the mechanism may be achieved by inhibiting the expression of OPA1 protein in the cells.

Key words

hypoxia/re-oxygenation / erythropoietin / optic atrophy 1 / renal proximal tubular cell / apoptosis

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LIAO Kai-nan,ZOU Li-le,DONG Wen-bin. Expression of optic atrophy 1 in renal tubular epithelial cells with hypoxia/re-oxygenation and the interventional effects of rhEPO[J]. Chinese Journal of Child Health Care. 2016, 24(2): 134-136 https://doi.org/10.11852/zgetbjzz2016-24-02-07

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