【Objective】 To detect the metabolite profiling and find the early biomarker or biomarkers in preterm infants with neonatal respiratory distress syndrome(NRDS) by a metabonomics technique based on 1H-NMR. 【Methods】 The preterm infants divided into 2 groups as NRDS group (NRDS) and pneumonia group(pneumonia) with 20 cases respectively. There was no difference in the age, gestational age and birth weight etc. Blood samples were drewed within 30 minutes after admission and the plasma were collected after centrifugalization. The plasma low-molecular mectabolites and lipid were detected. 【Results】 1)The concentrations of lactate, some lipid and alcohol were higher, the concentration of glucose, creatine, glutamine, OAc, NAc, alanine, valine, isoleucine were lower in NRDS group comparing to pneumonia group .2)The concentrations of HC=CH lipid, NAc, PtdCho, LDL and VLDL were lower in NRDS comparing to pneumonia. 【Conclusions】 Preterm infants with NRDS has lactate accumulation and lack the main energy substances like glucose and important amino acids with a lipid metabolic disturbance as while. Metabonomics has a great perspective in preterm infants diseases.
Key words
preterm infants /
neonatal respiratory distress syndrome /
metabonomics /
principal component analysis /
partial-leasts-quares-discriminant
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] Nicholson JK, Lindon JC, Holmes E. Metabonomics: understanding the metabolic responses of living systems to pathophysiological stimuli via multi-variate statistical analysis of biological NMR spectroscopic data[J]. Xenobiotica,1999,29(11):1181-1189.
[2] Waterman CL, Kian-Kai C, Griffin JL. Metabolomic strategies to study lipotoxicity in cardiovascular disease[J]. Biochim Biophys Acta,2010,1801(3):230-234.
[3] Lao YM, Jiang JG, Yan L. Application of metabonomic analytical techniques in the modernization and toxicology research of traditional Chinese medicine[J]. Br J Pharmacol,2009,157(7):1128-1141.
[4] Carraro S, Giordano G, Reniero F, et al. Metabolomics: A new frontier for research in pediatrics[J]. J Pediatr,2009,154(5):638-644.
[5] Zivkovic AM, German JB. Metabolomics for assessment of nutritional status[J]. Curr Opin Clin Nutr Metab Care,2009,12(5):501-507.
[6] 金汉珍,黄德氓,官希吉.实用新生儿学[M].3版.北京:人民卫生出版社,2004:422-423.
[7] Deshpande SA, Platt MP. Association between blood lactate and acid-base status and mortality in ventilated babies[J]. Archives of Disease in Childhood Fetal & Neonatal Edition,1997,76(1):15-20.
[8] Hofford JM, Milakofsky L, Pell S, et al. A profile of amino acid and catecholamine levels during endotoxin-induced acute lung injury in sheep: searching for potential markers of the acute respiratory distress syndrome[J]. Journal of Laboratory & Clinical Medicine,1996,128(6):545-551.
[9] Gore DC,Wolfe RR. Metabolic response of muscle to alanine, glutamine, and valine supplementation during severe illness[J]. JPEN,2003,27(5):307-314.
[10] Ahola T, Levonen A, Fellman V, et al. Thiol metabolism in preterm infants during the first week of life[J]. Scandinavian Journal of Clinical & Laboratory Investigation,2004,64(7):649-658.
[11] 刘喜红,王春枝,王蔚,等.早产儿呼吸窘迫综合征7种微量元素检测与分析[J].中国儿童保健杂志,2010,18(3):202-205.
PDF(482 KB)
