Lithium-mediate influence on stem cells in vitro culture in neonate rat in anaerobic condition

journal1 ›› 2013, Vol. 21 ›› Issue (10): 1045-1048.

Previous Articles Next Articles

Lithium-mediate influence on stem cells in vitro culture in neonate rat in anaerobic condition

WANG Yu^1,2,YIN Xiao-juan^1,2

1 Bayi Children's Hospital Affiliated to General Hospital of Beijing Military Command of People's Liberation Army,Beijing 100700,China; 2 Graduate School of Anhui Medical University,Institute of Beijing Military Region General Hospital,Hefei,Anhui 230001,China

Received:2013-03-13 Online:2013-10-06 Published:2013-10-06
Contact: YIN Xiao-juan,E-mail:yyinxiaojuan@163.com

锂对缺氧状态下新生鼠神经干细胞损伤的影响

王钰^1,2,尹晓娟^1,2

1 北京军区总医院附属八一儿童医院,北京 100700; 2 安徽医科大学北京军区总医院临床学院,安徽合肥 230001

通讯作者: 尹晓娟,E-mail:yyinxiaojuan@163.com
作者简介:王钰(1986-),女,山东人,现为安徽医科大学北京军区总医院临床学院在读硕士研究生,主要从事新生儿脑修复的临床与基础研究。

Abstract

Abstract: Obiective To establish hypoxia damage model of neural stem cells (NSCs) and investigate the protection of lithium on them through culture NSCs. Methods NSCs were cultured from neonatal mice,immunocytochemistry was used to examine the Nestin expression of NSC,the forth passage cells were divided into control,hypoxia and lithium groups,NSE and GFAP were used to evaluate cell differentiation,cck-8 was used to evaluate cell proliferation,and NSE and GFAP were used to evaluate cell differentiation. Result NSCs under hypoxia 95%N₂,5%CO₂ for 30 minutes could establish hypoxia damage model in vitro and certain concentration of lithium protected the damages. Conclusion Lithium can protect NSCs under hypoxia and may prevent NSCs against hypoxia injury.

Key words: neural stem cells, hypoxia, lithium

摘要： 目的培养神经干细胞,制造缺氧模型并探讨锂对缺氧损伤的神经干细胞是否具有保护作用。方法选取培养第四代的新生SD大鼠神经干细胞进行缺氧,细胞分为缺氧组和不缺氧组,每组内又分为不加锂组,加锂组(1、2、3、4、5mm)。免疫组织化学检测nestin、NSE和GFAP的表达。结果 95%N₂和5%CO₂混合气体培养细胞30min能成功构建离体神经干细胞缺氧损伤模型,一定浓度的锂能对缺氧损伤的神经干细胞发挥保护作用。结论锂对缺氧下神经干细胞有保护作用。

关键词: 神经干细胞, 缺氧, 锂

CLC Number:

Q426

WANG Yu,YIN Xiao-juan. Lithium-mediate influence on stem cells in vitro culture in neonate rat in anaerobic condition[J]. journal1, 2013, 21(10): 1045-1048.

王钰,尹晓娟. 锂对缺氧状态下新生鼠神经干细胞损伤的影响[J]. 中国儿童保健杂志, 2013, 21(10): 1045-1048.

References

[1] Pisani F,Orsini M,Braibani S,et al.Development of epilepsyin new-borns with moderate hypoxic-ischemic encephalopathy and neonatal seizures[J].Brain Dev,2009,31(1):64-68.
[2] 邵肖梅,叶鸿瑁,丘小汕.实用新生儿学[M].4版.2011:700-705.
[3] Li HF,Li Q,Du XN,et al.Lithium-mediated long-term neuroprotection in neonatal rat hypoxia-ischemia is associated with antiinflammatory effects and enhanced proliferation and survival of neural stem/progenitor cells[J].Journal of Cerebral Blood Flow & Metabolism,2011,31(10):2106-2115.
[4] 王钰,尹晓娟.锂对新生儿缺血缺氧性脑损伤神经保护作用的研究进展[J].中国儿童保健杂志,2011,19(11):1002-1003.
[5] 龚敏,李树清.体外培养细胞缺氧模型及特点[J].临床合理用药,2011,4(1):157-158.
[6] Tutunculer E.The prolecfive role of melalonin in experimental hypoxic brain damage[J].Pediatr Int,2005,47(4):434-439.
[7] Romanko MJ,Rothstein RP.Levis on SW Neural stem cells in the subventricular zone are resiIient to hypoxia / ischemia whereas progenitorsare vulnerable[J].J Cereb Blood Flow Metab,2004,24(7):814-825.
[8] Brazel CY,Rosti RT,Boyce S,et al.Perinatal hypoxia-ischemia damages and depletes progenitors from the mouse subventricular zone[J].Dev Neurosci,2004,26(2-4):266-274.
[9] Acker T,Acker H.Cellular oxygen sensing need in CNS function:physiological and pathological implications[J].JEXP Biol,2004,207(18):3171-3188. [10] Bian Q,Shi T,Chuang DM,et al.Lithium reduces ischemia-induced hippocampal CA1 damage and behavioral deficits in gerbils[J].Brain Res,2007,1184:270-276.
[11] Xia Y,Wang CZ,Liu J,et al.Lithium protection of phencyclidine-induced neurotoxicity in developing brain:the role of phosphatidylinositol-3 kinase/Akt and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase signaling pathways[J].J Pharmacol Exp Ther,2008,326:838-848.
[12] Crespo-Biel N,Camins A,Pallas M,et al.Evidence of calpain/cdk5 pathway inhibition by lithium in 3-nitropropionic acid toxicity in vivo and in vitro[J].Neuropharmacology,2009,56:422-428.
[13] Jin SK,Mi-Yoon C,In Tag Y,et al.Lithium selectively increases neuronal differentiation of hippocampal neural progenitor cells both in vitro and in vivo[J].Journal of Neurochemistry,2004,89:324-336.

[1]	GAO Shujun, ZHANG Hao, WU Muyang, ZHANG Xinyue, XIAO Peilun, WANG Xiaoli, WANG Fantao. Effects of hypoxic preconditioning of hDPSCs on autophagy and ATG7 in brain neurons of neonatal HIBD rats [J]. Chinese Journal of Child Health Care, 2023, 31(8): 851-857.
[2]	CHEN Chang-chun,ZHOU Qin,YAN Yan,WANG Qi,ZHANG Xiao-yuan,YIN Xiao-juan. Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells [J]. journal1, 2017, 25(2): 135-138.
[3]	LIAO Kai-nan,ZOU Li-le,DONG Wen-bin. Expression of optic atrophy 1 in renal tubular epithelial cells with hypoxia/re-oxygenation and the interventional effects of rhEPO [J]. journal1, 2016, 24(2): 134-136.
[4]	ZHANG Lan,TANG Jiu-lai.. Conductive education early intervention on hypoxia ischemia encephalopathy infants and correlation analysis between Peabody and Gesell Scales. [J]. journal1, 2015, 23(7): 730-732.
[5]	CHEN Hui-jun,YANG Ting-tong. Expression and significance of serum miRNA-21 control HIF-1α in newborn with asphyxia. [J]. journal1, 2015, 23(1): 32-34.
[6]	ZHU Di-qing,PANG Gao-feng. Effect of erythropoietin on vascular endothelial growth factor and nuclear factor kappa B expression in neonatal rats after hypoxic-ischemic brain damage. [J]. journal1, 2015, 23(1): 42-45.
[7]	ZHANG Xiao-li, JIA Tian-ming, DU Kai-xian, ZHAO Shan-shan, NIU Guo-hui. Role of L-thyroxine on brain tissue hypoxia-inducible factor 1α expression after hypoxia-ischemia brain damage in neonatal rats. [J]. journal1, 2014, 22(7): 702-704.
[8]	CUI Ying, LUAN Zuo, YANG Yin-xiang, WANG Cai-ying, GU Gui-xiong.. Effect of granulocyte-colony stimulating factor on survival of implanted human neural stem cells and the differentiation into oligodendrocytes. [J]. journal1, 2014, 22(2): 149-152.
[9]	WANG Yu, YIN Xiao-juan.. Cultivation, differentiation and identification in vitro of the neural stem cells from neonatal rat. [J]. journal1, 2014, 22(1): 31-34.
[10]	CHEN Hui-jun, YANG Yang.. Study on protective effect and its mechanism of pretreatment of Caspase-3 gene inhibiter on hypoxic-ischemic brain damage in the neonatal rats. [J]. journal1, 2014, 22(1): 38-40.
[11]	CHEN Yan,HOU Qing-hua,LU Chao,YE Min,YE Xiao-fen,ZHAO Chun-ling.. Effect of chronic intermittent hypoxia on renal ultrastructure and function in immature rats. [J]. journal1, 2013, 21(7): 708-710.
[12]	CHEN Yan,ZHAO Chun-ling,YU Guang,CHENG Ji-yan.. Effects of intermittent hypoxia on cognition and the ultrastructure of hippocampal synapses in immature rats. [J]. journal1, 2013, 21(6): 604-606.
[13]	CHANG Xiao,TANG Cheng-he,CAO Yin-li,SHANG Yun,SHI Ji-peng,JIN Jian-yun.. Changes in expressions of vascular endothelial growth factor and inducible nitric oxide synthase protein in neonatal rats with white matter damage. [J]. journal1, 2013, 21(3): 276-279.
[14]	MENG Shu-ying.. Predictive value of serum and urine S-100B protein for the outcome of neonatal hypoxic-ischemic encephalopathy. [J]. journal1, 2013, 21(2): 163-165.

Lithium-mediate influence on stem cells in vitro culture in neonate rat in anaerobic condition

锂对缺氧状态下新生鼠神经干细胞损伤的影响

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics