Metabonomics analysis of serum in autistic children using ultra performance liquid chromatography and Q-TOF mass spectrometry

SUN Meng; WANG Han; WANG Mao-qing; SUN Cai-hong; LIANG Shuang; ZOU Ming-yang; WU Li-jie; SUN Chang-hao.

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Chinese Journal of Child Health Care ›› 2013, Vol. 21 ›› Issue (1) : 6-9.

Metabonomics analysis of serum in autistic children using ultra performance liquid chromatography and Q-TOF mass spectrometry

SUN Meng¹,WANG Han^1,2,WANG Mao-qing³,SUN Cai-hong,LIANG Shuang¹,ZOU Ming-yang¹,WU Li-jie¹,SUN Chang-hao³.

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Abstract

Objective To investigate the metabolite alterations in the serum of autistic children and normal children using metabolomics profiling,discover specific biomarkers of autistic children,and provide experimental evidence for the early screening and diagnosis of children with autism. Method Ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC/Q-TOF MSMS) and pattern recognition were used to the Metabolomic Profiling of autistic children(n=73)and normal children (n=63),in order to discover the special metabolites. Results There were significant differences in serum metabolites of the autism group and control group within a total of 14 biomarkers in the positive and negative mode.Lysophosphatidic and sphingolipids increased in the autism group;Acylcarnitine and PUFAs reduced in the autism group. Conclusion There are significant differences in serum metabolites of children with autism and normal children that help to provide a basis for discovering potential markers useful for autism diagnosis,and provide new targets for autism intervention.

Key words

UPLC/Q-TOF MSMS / metabonomics / autism

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SUN Meng,WANG Han,WANG Mao-qing,SUN Cai-hong,LIANG Shuang,ZOU Ming-yang,WU Li-jie,SUN Chang-hao.. Metabonomics analysis of serum in autistic children using ultra performance liquid chromatography and Q-TOF mass spectrometry[J]. Chinese Journal of Child Health Care. 2013, 21(1): 6-9

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