Abstract: 【Objective】 To analyze the change in cell-mediated function in infants vaccinated with BCG. 【Methods】 Peripheral blood specimen from 150 infants with positive PPD test (Group successfully Vaccination,SV) and 50 ones with negative PPD test (Group failure vaccination,FV) were collected.The cytokine IFN-γ and IL-10 concentrations were measured by ELISA to evaluate cell-mediated immunity function after BCG vaccination. 【Results】 Serum IFN-γ concentrations in Groups SV and FV were (49.78±12.20)and (38.08±7.68)pg/mL,respectively(P=0.001).Serum IL-10 contents in Group SV and FV were (3.60±2.00) and (5.71±2.71)pg/mL,respectively (P<0.01). 【Conclusion】 The infants vaccinated successfully (positive PPD test) had an increasing cell-mediated immunity function.

Key words: bacillus calmette-guerin, cell-mediated immunity, cytokine

摘要： 【目的】 分析新生儿接种卡介苗(bacillus calmette-guerin,BCG)后细胞免疫功能变化。 【方法】 收集150例BCG接种后结核菌素纯蛋白衍生物(purified protein deriva-tive,PPD)阳性婴儿(接种成功组)和50例BCG接种后PPD阴性婴儿(接种失败组)的外周静脉血,应用ELISA方法测定血清细胞因子IFN-γ和IL-10的含量,评价细胞免疫功能。 【结果】 接种成功组和接种失败组血清细胞因子IFN-γ含量分别为(49.78±12.20)和(38.08±7.68)pg/mL,差异具有统计学意义(P=0.001);血清IL-10含量分别为(3.60±2.00)和(5.71±2.71)pg/mL,差异具有统计学意义(P<0.01)。 【结论】 BCG接种成功(PPD阳性)婴儿的细胞免疫功能增强。

关键词: 卡介苗, 细胞免疫, 细胞因子