Objective To evaluate the effect of iron deficiency on lymphocyte proliferation and cytokine secretion in children with iron deficiency anemia (IDA),and to explore the causes of the decline of immune function in children caused by IDA. Methods Totally 20 IDA children were selected in IDA group and 20 healthy children were selected as control group from March 4th to April 28th in 2018. The lymphocyte stimulating index under 10μmol/L,20 μmol/L and 50 μmol/L iron supplementation from IDA group and control group was examined by MTT. The production of INF-γ,IL-2,IL-6 and IL-10 by peripheral blood mononuclear cells (PBMC) under different iron supplementation was examined by ELISA. Results 1) In the control group,SI of B and T cells under iron concentration of 20 μmol/L was significantly higher than that in other iron concentration levels,and was also significantly higher than that under iron concentration of 20 μmol/L in IDA group. Moreover,the SI of IDA children was significantly increased after physiological concentration iron supplementation.2) PBMC from IDA patients showed significantly lower production of INF-γ and IL-2 at all iron concentration levels than PBMC from healthy controls,but INF-γ and IL-2 levels at the concentration of 20 μmol/L were significantly higher than those at the concentration of 10 μmol/L group.3) The secretory levels of IL-6 and IL-10 in IDA group were significantly higher than those in control group at the concentration of 10 μmol/L and 20 μmol/L. Conclusion The changes of T,B lymphocyte proliferation and cytokine secretion in IDA children may be one of the reasons for the imbalance of immune function and weakening immune system
Key words
iron deficiency anemia /
lymphocytes proliferation /
cytokine /
children
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References
[1] 卢良军,贾华芬.黄石市铁山区小学 6～12 岁儿童营养性缺铁性贫血发病因素调查及综合干预效果分析[J].中国妇幼保健,2017,32(4):836-839.
[2] 祝建忠,任海燕,王雪民.杭州市余杭区小儿缺铁性贫血现况调查及相关因素分析[J].实用预防医学,2014,21(4):471-473.
[3] El-Gendy FM,El-Hawy MA,Rizk MS,et al.Value of soluble transferrin receptors and sTfR/log ferritin in the diagnosis of iron deficiency accompanied by acute infection.[J].Indian J Hematol Blood Transfus,2018,34(1):104-109.
[4] 《中华儿科杂志》编辑委员会,中华医学会儿科学分会血液学组,中华医学会儿科学分会儿童保健学组.儿童缺铁和缺铁性贫血防治建议[J].中国儿童保健杂志,2010,18(8):724-726.
[5] Xu L,Meng Y,Liu Y,Meng Q,et al.A novel iron supplements preparation from Grifola frondosa polysaccharide and assessment of antioxidant,lymphocyte proliferation and complement fixing activities[J] .Int J Biol Macromol,2018,108:1148-1157.
[6] Gichohi-Wainaina WN,Towers GW,Swinkels DW, et al.Inter-ethnic differences in genetic variants within the transmembrane protease,serine 6 (TMPRSS6) gene associated with iron status indicators:a systematic review with meta-analyses[J].Genes Nutr,2015,10(1):442.
[7] Huang P,Wang J,Lin X,et al. Effects of IL-10 on iron metabolism in LPS-induced inflammatory mice via modulating hepcidin expression[J].Eur Rev Med Pharmacol Sci,2017,21(15):3469-3475.
[8] Mahajan G,Sharma S,Chandra J,et al.Hepcidin and iron parameters in children with anemia of chronic disease and iron deficiency anemia[J].Blood Res,2017,52(3):212-217.
[9] Song S,Yang L,Ye M,et al. Antioxidant activity of a Lachnum YM226 melanin-iron complex and its influence on cytokine production in mice with iron deficiency anemia[J].Food Funct,2016,7(3):1508-1514.
[10] Qiao X,Xie X,Shi W,et al. OCH-mediated shift of Th1 and Th2 cytokines by NKT cells in mice with aplastic anemia[J].Med Oncol,2015,32(3):67
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