【Objective】 Establish the precocious puberty model in female rats induced by excitatory amino acids receptor agonist. 【Methods】 Fifty 26-day-old female rats were randomly assigned to 5 groups, experimental groupⅠ(early phase of precocious puberty), the second experimental groupⅡ(metaphase of precocious puberty), control groupⅠ(normal prepuberty), control groupⅡ(early phase of normal puberty), control groupⅢ(metaphase of normal puberty). Study group received subcutaneous injection of N-methyl-DL-aspartate acid(NMA) twice a day until vaginal opening(VO) occurred and control groups received saline. The parameters of puberty were analyzed, which including index of ovary, index of uterus, incidence of corpora lutea, thickness of uterus and serum luteinizing hormone. 【Results】 The age of VO and first diestrus of experimental groups were earlier than the control groups. The parameters of puberty in experimental groups were different from control groupⅠ. The parameters of puberty in experimental groupⅠ were not different from control groupⅡ. The parameters of puberty in experimental groupⅡ were not different from control groupⅢ. 【Conclusions】 The precocious puberty model in female rats were successfully established by subcutaneous injection of NMA.
Key words
puberty /
precocious puberty /
excitatory amino acids /
N-methyl-DL-aspartate acid
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References
[1] Plant TM. The role of Kiss-1 in the regulation of puberty in higher primates[J]. Eur J Endocrinol,2006,155:11-16.
[2] Plant TM, Barker-Gibb ML. Neurobiological mechanisms of puberty in higher primates[J]. Hum Reprod Update,2004,10(1):67-77.
[3] Carbone S, Szwarcfarb B, Reynoso R, et al. Leptin stimulates LH secretion in peripubertal male rats through NMDA receptors[J]. EndocrRes,2005,31(4):387-396.
[4] Plant TM, Ramaswamy S, Dipietro MJ. Repetitive activation of hypothalamic G protein- coupled receptor 54 with intravenous pulses of kisspeptin in the juvenile monkey (Macaca mulatta) elicits a sustained train of gonadotropin- releasing hormone discharges[J]. Endocrinology,147(2):1007-1013.
[5] Terasawa E, Fernandez DL. Neurobiological mechanisms of the onset of puberty in primates[J]. Endocr Rev,2001,22(1):111-151.
[6] Clarkson J, Han SK, Liu X, et al. Neurobiological mechanisms underlying kisspeptin activation of gonadotropin-releasing hormone(GnRH) neurons at puberty[J]. Mol Cell Endocrinol,2010,324(1-2):45-50.
[7] Moguilevsky JA, Wuttke W. Changes in the control of gonadotrophin secretion by neuro- transmitters during sexual development in rats[J]. Exp Clin Endocrinol Diabetes,2001,109(4):188-195.
[8] Nyberg CL, Hiney JK, Minks JB, et al. Ethanol alters N-methyl-DL-aspartic acid-induced secretion of luteinizing hormone releasing hormone and the onset of puberty in the female rat[J]. Neuroendocrinology,1993,57(5):863-868.
[9] 田占庄,赵宏,陈伯英.达那唑诱导的雌性性早熟大鼠GnRH及其受体mRNA的表达[J].中华内分泌代谢杂志,2003,19(5):399-401.
[10] Morishita H, Takemoto M, Kondo H, et al. Induction of true precocious puberty by neonatal treatment with danazol in female rats[J]. Neurosci Lett,1993,157(1):33-36.
[11] Honaramooz A, Chandolia RK, Beard AP, et al. Excitatory amino acid regulation of gonadotropin secretion in prepubertal heifer calves[J]. Biol Reprod,1998,59(5):1124-1130.
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