Abstract: 【Objective】 Establish the precocious puberty model in female rats induced by excitatory amino acids receptor agonist. 【Methods】 Fifty 26-day-old female rats were randomly assigned to 5 groups, experimental groupⅠ(early phase of precocious puberty), the second experimental groupⅡ(metaphase of precocious puberty), control groupⅠ(normal prepuberty), control groupⅡ(early phase of normal puberty), control groupⅢ(metaphase of normal puberty). Study group received subcutaneous injection of N-methyl-DL-aspartate acid(NMA) twice a day until vaginal opening(VO) occurred and control groups received saline. The parameters of puberty were analyzed, which including index of ovary, index of uterus, incidence of corpora lutea, thickness of uterus and serum luteinizing hormone. 【Results】 The age of VO and first diestrus of experimental groups were earlier than the control groups. The parameters of puberty in experimental groups were different from control groupⅠ. The parameters of puberty in experimental groupⅠ were not different from control groupⅡ. The parameters of puberty in experimental groupⅡ were not different from control groupⅢ. 【Conclusions】 The precocious puberty model in female rats were successfully established by subcutaneous injection of NMA.

Key words: puberty, precocious puberty, excitatory amino acids, N-methyl-DL-aspartate acid

摘要： 【目的】 应用兴奋性氨基酸受体激动剂建立雌性大鼠性早熟模型。 【方法】 雌性SD大鼠50只,随机分为5组:实验Ⅰ组(性早熟青春早期组),实验Ⅱ组(性早熟青春中期组),对照Ⅰ组(正常青春前期组),对照Ⅱ组(正常青春早期组),对照Ⅲ组(正常青春中期组),实验组皮下注射N-甲基-D,L-天冬氨酸(NMA),对照组注射生理盐水,观察大鼠阴道开放时间及性周期,测量子宫指数、卵巢指数、卵巢黄体出现率、子宫壁厚度、黄体生成素浓度。 【结果】 实验组大鼠阴道开放及首次发情间期出现的时间较对照组提前,实验组各项指标较对照Ⅰ组有显著差异,实验Ⅰ组较对照Ⅱ组、实验Ⅱ组较对照Ⅲ组差异无统计学意义。 【结论】 应用兴奋性氨基酸受体激动剂可以使雌性大鼠正常的性发育提前,建立性早熟模型。

关键词: 青春期, 性早熟, 兴奋性氨基酸, N-甲基-D, L-天冬氨酸