【Objective】 To analyze the correlation between clinical characteristics, laboratory investigations and perinatal risk factors in neonates with sepsis. 【Method】 Retrospective study was conducted comparing the clinica profile of 67 neonatal sepsis patients and 75 uninfected patients admitted to the hospital 【Results】 Premature rupture of membranes for more than 18 hours, preterm infant low birth weight infant and fetus heart rate late deceleration were risk factors of neonatal sepsis. Theincidence of stagnant temperature or fever, poor response, diarrhea, abdominal distention, relapsing or exacerbating jaundice, neonatal scleredema and arrhythmia were significantly higher in the sepsis group than those in the controll group. The incidence of WBC≥20×10⁹or ≤5×10⁹, the ratio of rhabdocyte/granularcyte I/T cell≥0.2 ,PLT＜100×10⁹,CRP≥8 mg/L in sepsis group were significantly higher than those in the control group. 【Conclusions】 Premature rupture of membranes, preterm infant low birth weight infant and fetus heart rate late deceleration are risk factors of neonatal sepsis. Blood culture combining peripheral WBC, I/T, PLT, CRP may contribute to the early diagnosis of neonatal sepsis.