【Objective】 To probe into the preschoolers behavior problems influence factors in order to provide a basis for promoting children's behavior development. 【Methods】 464 preschoolers were investigated with self-designed family demographic information questionnaire, preschool temperament scale, 3~6 years old children family nurture environment scale, the teacher's questionnaire on children's self-confidence and children's behavior checklist. 【Results】 The boys had higher score than the girl about thinking problem and disciplinary problems. Children's retreat, aggression and external problem decreased with age. Children whose parent's occupation were executive staff had lower behavior score than others. Easy type of temperament children had lower behavior score than those difficulty type and slow-to-warm-up type. The family social adaptive, diverse activities and atmosphere factors were obviously correlated with the children's retreat, social, attention and aggression problems. The family ignorance/interference factor was correlated with anxiety depression, social, attention, aggression problems. Children's self-efficacy, self-expression scores had weak correlations with children's retreat behavior problems. 【Conclusion】 Children's behavior problems are influenced by individual internal biological factors such as gender, age, and psychological factor such as temperament, and external home nurture environment factors.