Association between maternal intestinal microbiota in the third trimester of pregnancy and infant food allergy

doi:10.11852/zgetbjzz2021-1749

Abstract

Abstract: Objective To investigate the relationship between maternal intestinal microbiota in the third trimester of pregnancy and infant food allergy, so as to provide new ideas for the association between microbial and food allergy. Methods A nested case-control study was adopted. Healthy pregnant women who had regular prenatal check-ups at Peking University Third Hospital from February 2018 to May 2020 and their newborns were selected to establish a maternal and infant cohort, of whom 135 mother-infant pairs met the criteria of this study and were followed up regularly. According to whether the infants had food allergy within 1 year old, 24 pairs were divided in the food allergy (FA) group and 44 pairs in the healthy control (HC) group. Fecal samples were collected from the mothers within 2 weeks before delivery and from the infants at 5 different time points after birth. These samples were analyzed by 16S rDNA sequencing. Results There was no significant difference in α diversity and β diversity of intestinal microbiota in the third trimester of pregnancy between the FA group and the HC group (P>0.05). Compared with the FA group, the relative abundance of Holdemania in maternal intestinal flora in the HC group was significantly increased(Z＝-2.103, P=0.035), while the relative abundance of Clostridium ⅪⅤa (Z＝-2.483, P=0.013), Clostridium Ⅳ (Z＝-2.154, P=0.031), Lactococcus (Z＝-2.147, P=0.032) and Parvimonas (Z＝-2.377, P=0.017) was significantly decreased. Among the intestinal microbiota of infants, there was no statistical significance in the difference between the main microbiota and the differential microbiota of maternal intestinal microbiota in late pregnancy at the same time point (P>0.05). Conclusions The relative abundance level of Holdemania in maternal intestinal microbiota in the third trimester of pregnancy is a potential predictor of food allergy in offspring, but is not associated with the relative abundance of Holdemania in offspring intestinal microbiota. Further studies on the mechanism of maternal intestinal microbiota in late pregnancy affecting offspring food allergy are warranted.

Key words: intestinal microbiota, the third trimester of pregnancy, infant, food allergy, 16S rDNA sequencing

摘要： 目的探讨母亲孕晚期肠道菌群与婴儿食物过敏的关系,为微生物与食物过敏的关系提供新方向。方法本研究为巢式病例-对照研究,选取2018年2月—2020年5月在北京大学第三医院妇产科规律产检的健康孕妇与其分娩的新生儿建立母婴队列,其中有135对母婴符合入排标准并规律随访,根据婴儿1岁以内是否出现食物过敏分为食物过敏(FA)组24对和健康对照(HC)组44对。收集母亲分娩前2周内的粪便标本以及婴儿生后5个不同时间点的粪便标本。采用16S rDNA基因测序对粪便标本进行微生物学分析。结果 FA组和HC组相比,母亲孕晚期肠道菌群在α多样性和β多样性上差异均无统计学意义(P>0.05)。HC组较FA组母亲晚期肠道菌群中霍尔德曼菌属(Z＝-2.103,P=0.035)相对丰度升高,而梭菌属ⅪⅤa群(Z＝-2.483,P=0.013)、梭菌属Ⅳ群(Z＝-2.154,P=0.031)、乳球菌属(Z＝-2.147,P=0.032)和细小单胞菌属(Z＝-2.377,P=0.017)相对丰度降低,差异均具有统计学意义。母亲孕晚期肠道菌群的主要菌属和差异菌属在婴儿肠道菌群中,在同一时间点上的差异无统计学意义(P>0.05)。结论母亲孕晚期肠道菌群中的霍尔德曼菌属相对丰度水平为子代婴儿食物过敏的潜在预测指标,且与子代婴儿肠道菌群中该菌的相对丰度水平无关。母体肠道菌群对后代食物过敏的影响机制有待进一步研究。

关键词: 肠道菌群, 孕晚期, 婴儿, 食物过敏, 16S rDNA测序

CLC Number:

R714

WANG Shuo, LI Xin-yue, WEI Yuan, LIU Lu-yan, LI Zai-ling, ZHANG Hua. Association between maternal intestinal microbiota in the third trimester of pregnancy and infant food allergy[J]. Chinese Journal of Child Health Care, 2022, 30(9): 941-946.

王硕, 李心悦, 魏瑗, 刘鹭燕, 李在玲, 张华. 母亲孕晚期肠道菌群与婴儿食物过敏的关系[J]. 中国儿童保健杂志, 2022, 30(9): 941-946.

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