Abstract: Objective To study the serum 25(OH)D levels of 0~7 years old children in Baoji,and to provide a scientific basis for taking vitamin D reasonable. Methods A total of 1 874 patients in Children's Health Department of Baoji Second People's Hospital were selected from January 2011 to June 2014.1 005 were male,869 were female.Using the high performance liquid chromatography - tandem mass spectrometry which was international advanced methods,serum 25(OH)D levels were determined of all the children. Results 1)The average level of serum 25(OH)D in 0～7 years old children in Baoji was (21.02±13.27) ng/ml,there were 996 children (53.15%) lake of serum 25(OH)D,390 children (20.81%) shortage in serum 25(OH)D,the value of serum 25(OH)D in zero month children was minimum,that in 3 years old children was maximum.2)The level of serum 25(OH)D was in ascending order form downtown,Chen-cang area,surrounding counties and the field.3)The levels of serum 25(OH)D in summer and autumn were significantly higher than those in spring and winter,and children's deficiency rate and inadequate rate of serum 25(OH)D in summer and autumn were significantly lower than those in spring and winter.4)The values of serum 25(OH)D in overweight and non-overweight children were (24.67±10.61)ng/ml and (20.11±11.57)ng/ml,the values of serum 25(OH)D in obese and non-obese children were (25.37±10.25)ng/ml and (20.56±10.74)ng/ml,there were significant differences in the serum 25(OH)D content between the two groups.5)Here was no significant difference in the serum 25(OH)D content between different sex. Conclusion The general lack of serum 25(OH)D exists in 0~7 years old children in Baoji area,the positive and effective measures should be taken for vitamin D supplement in 0~7 years old children.

Key words: 25-hydroxyvitamin D, high performance liquid chromatography-tandem mass spectrometry, 0~7 years old children

摘要： 目的 调查宝鸡地区0～7岁儿童血清25(OH)D水平,为0～7岁儿童合理摄入及补充维生素D提供科学依据。方法 选用2011年1月-2014年6月来宝鸡市第二人民医院儿童保健科体检儿童1 874例。其中男1 005例,女869例,采用国际先进的高效液相色谱-串联质谱法测定所有研究对象的血清25(OH)D水平。结果 1)宝鸡市0～7岁儿童血清25(OH)D均值为(21.02±13.27)ng/ml,996例(53.15%)存在血清25(OH)D缺乏,390例(20.81%)存在血清25(OH)D不足,0月～组最低,3岁～组最高。 2)市区(金台、渭滨)、陈仓区、周边县区、外地血清25(OH)D水平依次递增。 3)夏季和秋季的血清25(OH)D水平显著高于春季和冬季,且夏、秋季儿童血清25(OH)D缺乏、不足率显著低于春、冬季。4)超重与非超重儿童血清25(OH)D分别为(24.67±10.61)ng/ml和(20.11±11.57)ng/ml,而肥胖与非肥胖儿童血清25(OH)D分别为(25.37±10.25)ng/ml和(20.56±10.74)ng/ml,组间血清25(OH)D差异均有统计学意义。5)不同性别血清25(OH)D差异无统计学意义。结论 宝鸡市0～7岁儿童血清25(OH)D普遍缺乏,应采取积极、有效的措施加强0～7岁儿童维生素D的摄入和补充。

关键词: 25羟维生素D, 高效液相色谱-串联质谱法, 0～7岁儿童