Objective To investigate the levels and deficience of 25-hydroxyvitamin D [25-(OH)D]in children at 3～6 years old. Methods Two kindergarten which have more than 200 children in each district were chosen,in total of ten kindergarten.a survey about vitamin D was conducted,the parents informed choice on the basis of the investigation.All tests were done in April to June 2013. Results A total of 753 cases of 3 ~ 6 years old children in the detection of vitamin D,183 cases (24.3%) were relative sufficient,360 cases (47.8%) were relative deficient;207 cases (27.5%) were absolutely deficient.There were statistical differences between different age groups in the deficience of vitamin D(P＜0.05)There Was no statistical difference between different gender groups(P>0.05).In these children,only 11.6% of the children drank milk more than 500ml every day,75.8% of the children didn't take any calcium and cod liver oil for more than one month,33.5% of the children's activity time was up to 1 hour after school. Conclusion The deficiency rate of 25-(OH)D might be high in children of 3～6 years old in Chengdu,we need to do something to improve the level of vitamin D.