Abstract: Objective To explore the effects of prenatal vibration stress in different gestational time points on the spatial learning and memory in rats. Methods The Sprague-Dawley (SD) pregnant rats were vibrated strong and weak alternately in 1~7 d,8~14 d,15~21 d of gestation period,and the offspring was tested in Barnes maze to identify their spatial learning and memory,the difference of each group then compared. Results Learning process:1)The journey of exploration:It was higher than control group during early pregnancy group was in 4 d,during middle pregnancy group in 1d and during late pregnancy group in 1d;It was lower than control group during late pregnancy group in 2 and 3 d (P＜0.05);2)The average speed of exploration:It was lower than control group during early pregnancy group in 2 d,during middle pregnancy group in 1 d and 2 d,during late pregnancy group in 1,2,3,7,8 d (P＜0.05);3)The time of exploring errors holes:It was higher than control group during early pregnancy group in 1,2,4,7,8 d,during middle pregnancy group in 3,4,5,8d;It was lower than control group during late pregnancy group in 2,6 d (P＜0.05);4)The latency of exploring target hole:It was higher than control group during middle pregnancy group in 5 d (P＜0.05).Memory process:The time of exploring errors holes was higher during early pregnancy group(Mann-Whitney U=43.00,P=0.000);The journey of exploration (Mann-Whitney U=150.00),the average speed of exploring (Mann-Whitney U=150.00) and the time of exploring errors holes (Mann-Whitney U=164.50) were lower during late pregnancy group(P<0.05 or <0.01). Conclusion The vibration stress in early pregnancy group may damage the spatial learning and memory ability,and it may promote the spatial learning and memory ability in the late pregnancy group; but the vibration stress in mid pregnancy group only damage the spatial learning ability.

Key words: prenatal vibration stress, Barnes maze, spatial learning and memory

摘要： 目的 探讨不同妊娠期振动对大鼠子代空间学习记忆的影响。方法 分别在SD大鼠妊娠的1~7 d、8~14 d、15~21 d施加强弱交替的振动刺激,在子鼠60 d使用Barnes 迷宫测试其空间学习记忆能力,比较各组间空间学习记忆的差异。结果 学习过程:1)探索路程:妊娠早期振动组在第4天,妊娠中期在第1天,妊娠晚期在第1天高于对照组,妊娠晚期在第2、3天低于对照组(P＜0.05);2)探索平均速度:妊娠早期在第2天,妊娠中期在第1、2天,妊娠晚期在第1、2、3、7、8天均低于对照组(P＜0.05);3)错误探索时间:妊娠早期在第1、3、4、7、8天,妊娠中期在第3、4、5、8天高于对照组,妊娠晚期在第2、6天低于对照组(P＜0.05);4)首次探索目标洞潜伏期:妊娠中期在第5天高于对照组(P＜0.05)。记忆过程:妊娠早期振动组的错误探索时间明显高于对照组(Mann-Whitney U=43.00,P=0.000),妊娠晚期振动组的探索路程(Mann-Whitney U=150.00)、平均速度(Mann-Whitney U=150.00)和错误探索时间(Mann-Whitney U=164.50)明显低于对照组(P<0.05或<0.01)。结论 妊娠早期振动损害子代的空间学习记忆能力,妊娠晚期振动可能促进子代的空间学习记忆能力,而妊娠中期仅损害子代的空间学习能力。

关键词: 妊娠期振动, Barnes迷宫, 空间学习记忆能力