Correlation of distribution of c-fos and neuropetdides in lung of asthmatic rat.

doi:10.11852/zgetbjzz2014-22-07-13

Abstract

Abstract: Objective To investigate the change of distribution of c-fos and neuropetides in the lung of asthmatic rat. Methods Thirty-two Wistar rats were divided into control group, asthmatic recover group, acute episode group, dexameth intervention group.Immunohistochemistry and computer image analysis were used to observe the c-fos change of the lung and the contents of substance P(SP) and vasoactive intestinal peptide(VIP) in lung tissues were detected by ELISA. Results The contents of SP in the BALF of asthmatic recover group, acute episode group, dexameth intervention group rats were significantly higher than that in control group(P＜0.000 1), and the contents of VIP were significantly lower.There were significantly positive correlations between the SP and the optical density value of c-fos(r=0.908, 0.967, 0.865, P<0.05 or <0.001).There were significantly negative correlations between VIP and optical density value of c-fos (r=-0.974, -0.949, -0.962, P all ≤0.001). Conclusion There is correlation between neuropeptides, c-fos proteinum and asthmatic attack asthmatic rats.

Key words: bronchial asthma, c-fos proteinum, neuropeptide

摘要： 目的探讨支气管哮喘大鼠肺内c-fos蛋白表达和神经肽P物质(substance P, SP)和血管活性肠肽(vasoactive intestinal peptide, VIP)含量及分析之间的相关性。方法 32只SD大鼠随机分成正常对照组、哮喘非急性发作期组、哮喘急性发作组和地塞米松干预组。采用免疫组织化学进行组织学观察, 采用酶联免疫吸附试验法测定肺泡灌洗液中SP和VIP的浓度。结果哮喘大鼠肺泡灌洗液中SP浓度较正常对照组显著增高(P＜0.000 1), VIP浓度显著降低(P＜0.000 1), 哮喘恢复组、哮喘急性期发作组及地塞米松干预组肺组织中c-fos蛋白光密度值与肺泡灌洗液SP浓度呈正相关(r分别为0.908、0.967、0.865, P<0.05或<0.001), 与肺泡灌洗液VIP浓度呈负相关(r分别为-0.974、-0.949、-0.962, P均≤0.001)。结论哮喘大鼠肺内c-fos蛋白表达、神经肽含量与哮喘发作有关。

关键词: 支气管哮喘, c-fos蛋白, 神经肽

CLC Number:

R256.12

LIU Hai-yan, HOU Wei, YANG Xu-dong, ZONG Chang-hong. Correlation of distribution of c-fos and neuropetdides in lung of asthmatic rat.[J]. journal1, 2014, 22(7): 709-712.

刘海燕, 侯伟, 杨旭东, 宗长红. 支气管哮喘大鼠肺内c-fos蛋白表达和神经肽含量相关性研究[J]. 中国儿童保健杂志, 2014, 22(7): 709-712.

References

[1] Demoly P, Basset Seguin N, Chanez P, et al.e-fos prote-oncogene expression in bronchial biopsies of asthmatics[J].Am Respir Cell Mol Biol, 1992, 7:128-133.
[2] van Beveren C, van Straaten F, Curran T, et al.Analsis of FBJ-MuSV provirus and c-fos(mouse)gene reveals that viral and cellular fos geng products have different carboxy termini[J].Cell, 1983, 32:1241-1255.
[3] Sacar SM, Sharp FR.Early response genes as markers of neuronal activity and growth factor action[J].Adv Neurol, 1993, 59:273-284.
[4] 李凝, 神经肽和哮喘[J].国外医学·呼吸系统分册, 2002, 22(1):45-48.
[5] 龙静雯, 马卓, 杨旭东, 等.不同品系哮喘大鼠哮喘模型比较[J].西安交通大学学报:医学版, 2009, 30(5):550-553.
[6] 应延风, 胡野, 盛秀胜, 等.大鼠哮喘模型中嗜酸性粒细胞和IL-10改变研究[J].现代中西医结合杂志, 2008, 17( 24):3744-3746.
[7] 尚云晓, 张晗, 叶丽红, 等.感觉神经肽在不同程度哮喘时对肺组织中含量变化及相关研究[J].小儿急救医学, 2004, 11(1):16-17.
[8] Jonas AE, Lore I.Human c-fos proto-oncogene mappel to chromosom 14, band q24.3-q31 possibilities for oncogene activation by chromosomal rearrangements in human neoplasma[J].Exp Cell Res, 1987, 169:262-265.
[9] Peter A, Michael K.The role of Jun fos and the AP-1 complex in cellproliferation and transformation[J].Biochem Biophys Acta, 1991, 1072:129-157.
[10] Panettieri RA, Yadbish Pa, Kelly AM, et al.Histamine stimulates proliferation of airway smooth muscle and induces c-fos expression[J].Am J Physiol, 1990, 259:365-371.
[11] Demoly P, Chanez P, Pujol JC, et al.Fos immunoreatibity assessment on human normal and pathological bronchial biopsies[J].Respiratory Medicine, 1995, 89:329-335.
[12] Tremblay L, Valenza F, Ribeiro SP, et al.Injurious ventilatory strategies increase cytokines and c-fos mRNA expression in an isolated rat lung modal[J].J Clin Invest, 1997, 99:994-952.
[13] O'connor TM, O'connell I, Obreen DI, et al.The Role of substance P in inflammatory disease[J].J Cell Physiol, 2004, 201:167-180.
[14] Joos GF, Geimonpre PR, Pauwels RA.Role of tachykinins in asthma[J].Allergy, 2000, 55:321-337.
[15] Groneberg DA, Quarcoo D, Frossard N, et al.Neurogenic mechanisms in bronchinal in flammatory diseases[J].Allergy, 2004, 59:1139-1152.
[16] 俞茹云, 王彤, 殷凯生, 等.支气管哮喘大鼠肺内非肾上腺素能非胆碱能神经分布及神经肽含量的相关性研究[J].中华哮喘杂志, 2009, 3(3):185-188.