Objective To explore the relationship between CTLA-4 gene polymorphism and susceptibility to bronchial asthma in children and peripheral blood IFN-γ, IL-4 and IgE, and provide theoretical and clinical basis for the diagnosis and treatment of bronchial asthma in children. Methods From April 2017 to February 2019,78 children with bronchial asthma in the Third Affiliated Hospital of Zhengzhou University were in the case group, and 67 healthy children in the physical examination were used as the control group.The rs231725 single nucleotide polymorphism of CTLA-4 gene was detected in both groups, and the levels of IFN-γ, IL-4 and IgE in peripheral blood of the case group were also detected. Results The proportion of CTLA-4 rs231725 AA genotype was higher in the case group than in the control group (66.7% vs 19.4%), and the proportion of CTLA-4 rs231725 GG genotype was lower than that in the control group (19.3% vs 73.2%) (P<0.05); CTLA-4 rs231725 A allele frequency ratio was higher than in the control group (73.7% vs 23.1%) (P＜0.05), and the CTLA-4 rs231725 G allele frequency ratio was lower than in the control group (26.3% vs 76.9%) (P <0.05); CTLA-4 rs231725 AG genotype IFN-γ, IL-4, IgE levels were higher than GG genotype, and the differences were statistically significant (P<0.05); At CTLA-4 rs231725 genotypes and allele frequencies, there were no significant correlation between bronchial asthma and urinary system damage, bronchial asthma with digestive tract reaction (P>0.05).CTLA-4 rs231725 AA genotype(OR=2.625,95%CI=1.273-5.138) and A allele(OR=1.987,95%CI=1.121-3.397) frequencie were associated with bronchial asthma with nephritis(P<0.05). Conclusion CTLA-4 rs231725 AA genotype and A allele are associated with increased susceptibility to bronchial asthma in children, which may be caused by the high levels of IFN-γ, IL-4, and IgE.
Key words
CTLA-4 gene polymorphism /
bronchial asthma /
susceptibility /
IFN-γ /
IL-4 /
IgE
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