关键词: 神经管畸形, Bcl-2, Bax, Caspase-3

Abstract: Objective To explore the molecular mechanism of oyster antagonistic neuronal apoptosis in neural tube defects(NTDs) caused by retinoic acid(RA). Methods NTDs model was set up in mouse embryo by overdose RA and was given the antagonists of oyster.The apoptosis situation and expression changes of apoptosis related proteins Bcl-2,Bax and Caspase-3 in neural epithelial cells of mouse embryo were observed. Results 1)The apoptotic index of neuroepithelial cells in RA group was significantly higher than that in the control group(P＜0.05).The apoptotic index of neuroepithelial cells in oyster group was significantly lower than that in RA group(P＜0.05).2)Bcl-2 expression of neuroepithelial cells in RA group was significantly lower than that in the control group(P＜0.05).Bcl-2 expression in oyster group was significantly higher than that in the RA group(P＜0.05).3)Bax and Caspase-3 expression on neur epithelial cells in RA group were significantly higher than that in the control group(P＜0.05).Bax and Caspase-3 expression in oyster group was significantly lower than that in the RA group(P＜0.05). Conclusion Oysters can antagonize neur epithelial cells excessive apoptosis in NTDs caused by RA.Its molecular mechanism may be realized through the up-regulation of Bcl-2 expression and down-regulation of Bax and Caspase-3 expression.

Key words: neural tube defects, Bcl-2, Bax, Caspase-3