关键词: 柯萨奇B3病毒, 病毒性心肌炎, IGF-1, 藻蓝蛋白

Abstract: Objective To investigate the effect of phycocyanin on the expression of insulin like growth factor-1 (IGF-1) in coxsackievirus B3(CVB3) mice myocarditis. Methods A total of 55 BalB/c mice were assigned into three groups:normal control group (group A,n=15),virus control group (group B,n=20),phycocyanin intervention group (group C,n=20).Mice were killed on the 15th day after inoculated,and the samples of blood and myocardium were obtained.Optical microscope was used to detect the inflammation and necrosis in myocardium.The level of ICF-1 in plasma was measured using ELISA.The expression of IGF-1 in myocardium was determined by RT-PCR and Real-time PCR. Results The survival rate of group C was higher than that of group B (P<0.05).Compare to group A,group B had higher level of ICF-1 in plasma and the higher expression of IGF-1 in myocardium.The level of ICF-1 in plasma,the histopathologic score and the higher expression of IGF-1 in myocardium of group C were lower than those of group B,but higher than group A (P<0.05). Conclusion Phycocyanin may influence the expression of ICF-1 and possibly thus providing protective effects on myocardium.

Key words: coxsackievirus B3, viral myocarditis, insulin like growth factor-1, phycocyanin