目的 研究双歧杆菌对坏死性小肠结肠炎(necrotizing enterocolitis,NEC)模型新生SD大鼠肠组织TNF-α及IL-10水平的影响,探讨双歧杆菌对NEC的保护作用。方法 2日龄SD大鼠128只随机分成LPS组(A组)、LPS+双歧杆菌组(B组)、生理盐水对照组(C组)、双歧杆菌对照组(D组),一周后,腹腔注射LPS后2、6、12、24 h断头处死大鼠,解剖留取肠管,其中回盲部近端肠管行病理学检查,剩余肠管ELISA法检测TNF-α、IL-10水平。结果 B组2、6、12、24 h肠组织病理损伤评分均显著低于A组(P<0.05)。B组2、6、12肠组织TNF-α水平均显著低于A组(P<0.05);B组2、6、12、24 肠组织IL-10水平均显著高于A组(P<0.05)。统计学表明:肠组织病理损伤评分与TNF-α水平呈正相关性(r=0.646),肠组织病理损伤评分与IL-10水平呈负相关性(r=-0.598)。 结论 双歧杆菌能抑制NEC模型中TNF-α水平和提升IL-10水平,对NEC具有保护作用。
Abstract
Objective To research bifidobacterium's effects on tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in intestinal tissue of neonatal SD rat model of necrotizing enterocolitis (NEC) and to investigate the protective effects of bifidobacterium against NEC. Methods 128 neonate Sprague-Dewley (SD) rats aged 2 days were divided into four groups at random:LPS group(A),LPS+bifidobacterium group(B),normal saline group (C) and bifidobacterium group(D).After received injection of LPS for a week,eight rats,which were selected form each group at random,were killed quickly respectively at 2,6,12 and 24 hours through decollation.The intestinal tissue obtained,the ileocecal junction was for histological analysis,and the remains was used to measure the level of TNF-α and IL-10 through ELISA method. Results Compared to group A,the scores of intestinal histologic pathological injury of group B at 2,6,12,24 hours were significantly lower (P<0.05) and the levels of TNF-α in ileal tissue of group B at 2,6,12 hours were significantly lower(P<0.05).Also compared to group A,the levels of IL-10 in ileal tissue of group B at 2,6,12,24 hours were significantly higher (P<0.05).Statistics showed that TNF-α level had positive correlation with the scores of neonatal rats of intestinal histologic injury (r=0.646) and IL-10 level had negative correlation with the scores of neonatal rats of intestinal histologic injury (r=-0.598). Conclusion Bifidobacterium could reduce the level of TNF- α and increase the level of IL-10 of NEC model,bifidobacterium provides protection from NEC.
关键词
双歧杆菌 /
坏死性小肠结肠炎 /
内毒素 /
肿瘤坏死因子 /
白介素10
Key words
bifidobacterium /
necrotizing enterocolitis /
LPS /
TNF-α /
IL-10
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 周景.冯宗太,查伟峰,等.预防性应用益生菌对极低出生体重儿坏死性小肠结肠炎影响的Meta分析[J].中国中医药咨讯,2010,2(34):64-67.
[2] De Vrese M,Schrezenmeir J.Bifidobacterium,prebiotics,and synbiotics[J].Food Biotechnology,2008,111(1):1-66.
[3] Henry MC,Moss LR.Necrotizing enterocolitis[J].Annu Rev Med,2009,60(1):111-124.
[4] Martin CR,Walker WA.Probiotics:role in pathophysiology and prevention in necrotizing enterocolitis[J].Semin Perinato,2008,32(2):127-137.
[5] Jarvis MA,Borton JA,Keech AM,et al.Human cytomegalovirus attenuates interleukin-1beta and tumor necrosis factor alpha proinflammatory signaling by inhibition of NF-kappaB activation[J].Journal of Virology,2006,80(11):5588-5589.
[6] Zwerina J,Redlich K,Polzer K,et al.TNF- induced structural joint damage is mediated by IL-1[J].Proceedings of the National Academy of Sciences,2007,104(28):11742-11747.
[7] Montag C,Wagner J,Gruska I,et al.Human cytomegalovirus blocks tumor necrosis factor-alpha and Interleukin-1beta-mediated NF-kappaB signaling[J].Journal of Virology,2006,80(23):11686-11689.
[8] Baud V,Karin M.Signal transduction by tumor necrosis factor and its relatives[J].Trends in Cell Biology,2001,11(9):372-377.
[9] Halpern MD,Clark JA,Saunders TA,et al.Reduction of experimental necrotizing enterocolitis with anti-TNF-α alpha[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,2006,290(4):757-764. [10] 黄柏枝,刘仿.双歧杆菌防治极低出生体重儿坏死性小肠结肠炎的作用研究[J].中国医药导报,2011,8(11):35-39.
[11] Halpern MD,Holubec H,Dominguez JA,et al.Up-regulation of IL-18 and IL-12 in the ileum of neonatal rats with necrotizing enterocolitis[J].Pediatric Research,2002,51(6):733-739.
[12] Ebach DR,Riehl TE,Stenson WF.Opposing effects of tumor necrosis factor receptor 1 and 2 in sepsis due to cecal ligation and puncture[J].Shock,2005,23(4):311-318.
[13] Tan XD,Tan XD,Chang H,et al.PAF increases mucosal permeability in rat intestine via tyrosine pho-sphorylation of ecadherin[J].Br J Phaxlnacol,2000,129:1522-1529.
[14] Bellinghausen I,Knop J,Saloga J.The role of interleukin 10 in the regulation of allergic immune responses[J].International Archives of Allergy and Immunology,2000,126(2):97-101.
[15] O'Donnell R,Breen D,Wilson S,et al.Inflammatory cells in the airways in COPD[J].British Medical Journal,2006,61(5):448-454.
PDF(475 KB)
