双歧杆菌对坏死性小肠结肠炎新生鼠模型肠损伤的保护

摘要/Abstract

摘要： 目的研究双歧杆菌对坏死性小肠结肠炎(necrotizing enterocolitis,NEC)模型新生SD大鼠肠组织TNF-α及IL-10水平的影响,探讨双歧杆菌对NEC的保护作用。方法 2日龄SD大鼠128只随机分成LPS组(A组)、LPS+双歧杆菌组(B组)、生理盐水对照组(C组)、双歧杆菌对照组(D组),一周后,腹腔注射LPS后2、6、12、24 h断头处死大鼠,解剖留取肠管,其中回盲部近端肠管行病理学检查,剩余肠管ELISA法检测TNF-α、IL-10水平。结果 B组2、6、12、24 h肠组织病理损伤评分均显著低于A组(P<0.05)。B组2、6、12肠组织TNF-α水平均显著低于A组(P<0.05);B组2、6、12、24 肠组织IL-10水平均显著高于A组(P<0.05)。统计学表明:肠组织病理损伤评分与TNF-α水平呈正相关性(r=0.646),肠组织病理损伤评分与IL-10水平呈负相关性(r=-0.598)。结论双歧杆菌能抑制NEC模型中TNF-α水平和提升IL-10水平,对NEC具有保护作用。

关键词: 双歧杆菌, 坏死性小肠结肠炎, 内毒素, 肿瘤坏死因子, 白介素10

Abstract: Objective To research bifidobacterium's effects on tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in intestinal tissue of neonatal SD rat model of necrotizing enterocolitis (NEC) and to investigate the protective effects of bifidobacterium against NEC. Methods 128 neonate Sprague-Dewley (SD) rats aged 2 days were divided into four groups at random:LPS group(A),LPS+bifidobacterium group(B),normal saline group (C) and bifidobacterium group(D).After received injection of LPS for a week,eight rats,which were selected form each group at random,were killed quickly respectively at 2,6,12 and 24 hours through decollation.The intestinal tissue obtained,the ileocecal junction was for histological analysis,and the remains was used to measure the level of TNF-α and IL-10 through ELISA method. Results Compared to group A,the scores of intestinal histologic pathological injury of group B at 2,6,12,24 hours were significantly lower (P<0.05) and the levels of TNF-α in ileal tissue of group B at 2,6,12 hours were significantly lower(P<0.05).Also compared to group A,the levels of IL-10 in ileal tissue of group B at 2,6,12,24 hours were significantly higher (P<0.05).Statistics showed that TNF-α level had positive correlation with the scores of neonatal rats of intestinal histologic injury (r=0.646) and IL-10 level had negative correlation with the scores of neonatal rats of intestinal histologic injury (r=-0.598). Conclusion Bifidobacterium could reduce the level of TNF- α and increase the level of IL-10 of NEC model,bifidobacterium provides protection from NEC.

Key words: bifidobacterium, necrotizing enterocolitis, LPS, TNF-α, IL-10

中图分类号:

Q426

蔺小培,顾志成. 双歧杆菌对坏死性小肠结肠炎新生鼠模型肠损伤的保护[J]. 中国儿童保健杂志, 2013, 21(4): 370-373.

LIN Xiao-pei,GU Zhi-cheng.. Protection of bifidobacterium from damage to neonatal rat model of necrotizing enterocolitis.[J]. journal1, 2013, 21(4): 370-373.

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