肥胖男童尿液差异代谢物的筛选

doi:10.11852/zgetbjzz2024-0654

中国儿童保健杂志 ›› 2025, Vol. 33 ›› Issue (2): 180-184.DOI: 10.11852/zgetbjzz2024-0654

肥胖男童尿液差异代谢物的筛选

刘韦^1*, 董洁^2*, 高超男¹, 齐千瑾¹, 孔亚伟¹, 孟鑫¹, 彭晓霞³, 闫银坤¹

1.首都医科大学附属北京儿童医院慢病中心,北京 100045;
2.湖南省儿童医院儿科医学研究所,湖南长沙 410000;
3.首都医科大学附属北京儿童医院临床流行病与循证医学中心

收稿日期:2024-06-20 修回日期:2024-09-11 发布日期:2025-02-12 出版日期:2025-02-10
通讯作者: 彭晓霞,E-mail:pengxiaoxia@bch.com.cn;闫银坤,E-mail:ykyan2011@163.com
作者简介:刘韦(1994—),女,本科生,主要研究方向为儿童慢性病流行病学;董洁(1991—),女,博士研究生,助理研究员,主要研究方向为儿童食盐与血压、菌群。注:^*共同第一作者。
基金资助:
北京新星计划(Z201100006820008);湖南省自然科学基金(2022J40198);国家自然科学基金(81803254, 81973110, 82073572)

Screening of differential metabolites in the urine of obese boys

LIU Wei^1*, DONG Jie^2*, GAO Chaonan¹, QI Qianjin¹, KONG Yawei¹, MENG Xin¹, PENG Xiaoxia³, YAN Yinkun¹

1. Center for Non-communicable Disease Management, Beijing Children′s Hospital, Capital Medical University, National Center for Children′s Health, Beijing 100045, China;
2. Pediatric Medical Research Institute, Hunan Children′s Hospital, Changsha, Hunan 410000, China;
3. Center for Clinical Epidemiology and Evidence-Based Medicine, BeijingChildren′s Hospital, Capital Medical University, National Center for Children′s Health

Received:2024-06-20 Revised:2024-09-11 Online:2025-02-10 Published:2025-02-12
Contact: PENG Xiaoxia, E-mail: pengxiaoxia@bch.com.cn; YAN Yinkun, E-mail: ykyan2011@163.com

摘要/Abstract

摘要： 目的探究肥胖男童与体重正常男童之间尿液代谢物特征的差异,为肥胖的发病机制提供初步线索。方法采用病例对照研究设计,于2021年9—10月在湖南长沙三所寄宿学校招募6~14岁儿童,按体重状态分为肥胖组和体重正常组,将两组儿童按年龄1∶1进行匹配,最终纳入10名正常体重男童和10名肥胖男童。连续收集3d 24h尿样,混匀后取2mL尿样进行靶向检测。采用正交偏最小二乘法判别分析比较两组间代谢物的整体组成差异、t检验和差异倍数值相结合的方法筛选差异代谢物。结果肥胖组中吲哚-3-甲基乙酸酯、吲哚-3-丙酸、吲哚乙酸、胆酸、乙酰胆碱酸、石胆酸-3-硫酸盐、7-酮去氢胆酸、甲基半胱氨酸、脯氨酸和癸二酸的水平低于正常体重组,差异有统计学意义(Log₂FC≤-0.5,P<0.05)。结论肥胖男童与体重正常男童的尿液代谢物存在差异,可为肥胖的预防和控制提供新线索。

关键词: 肥胖, 差异代谢物, 尿液, 代谢组学

Abstract: Objective To investigate the differences in urinary metabolite profiles between obese and normal-weight boys, in order to provide preliminary clues for understanding the pathogenesis of obesity. Methods A case-control study design was employed. From September to October 2021, children aged 6 - 14 years were recruited from three boarding schools in Changsha, Hunan. Participants were divided into obese and normal-weight groups based on their weight status. Ten normal-weight boys and ten obese boys were matched by age(1∶1 ratio).24-hour urine samples were collected continuously for three days, mixed, and 2mL of urine was taken for targeted detection. Orthogonal partial least squares discriminant analysis(OPLS-DA) was used to compare the overall differences in metabolite composition between the two groups, and a combination of t-test and fold change(FC) values was employed to screen for differential metabolites. Result The levels of Indole-3-methyl acetate, Indole-3-propionic acid, Indoleacetic acid, Cholic acid, Glycocholic acid, Sulfated lithocholic acid, 7-dehydrocholic acid, Methylcysteine, Proline, and Sebacic acid were significantly lower in the obese group compared to the normal-weight group(Log₂FC≤-0.5, P <0.05). Conclusion Differences in urine metabolites exist between obese and normal-weight male children, which may provide new clues for the prevention and control of obesity.

Key words: obesity, differential metabolites, urine, metabolomics

中图分类号:

R723.14

刘韦, 董洁, 高超男, 齐千瑾, 孔亚伟, 孟鑫, 彭晓霞, 闫银坤. 肥胖男童尿液差异代谢物的筛选[J]. 中国儿童保健杂志, 2025, 33(2): 180-184.

LIU Wei, DONG Jie, GAO Chaonan, QI Qianjin, KONG Yawei, MENG Xin, PENG Xiaoxia, YAN Yinkun. Screening of differential metabolites in the urine of obese boys[J]. Chinese Journal of Child Health Care, 2025, 33(2): 180-184.

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肥胖男童尿液差异代谢物的筛选

Screening of differential metabolites in the urine of obese boys

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