肥胖男童尿液差异代谢物的筛选

刘韦; 董洁; 高超男; 齐千瑾; 孔亚伟; 孟鑫; 彭晓霞; 闫银坤

doi:10.11852/zgetbjzz2024-0654

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中国儿童保健杂志 ›› 2025, Vol. 33 ›› Issue (2) : 180-184. DOI: 10.11852/zgetbjzz2024-0654

科研论著

肥胖男童尿液差异代谢物的筛选

刘韦^1*, 董洁^2*, 高超男¹, 齐千瑾¹, 孔亚伟¹, 孟鑫¹, 彭晓霞³, 闫银坤¹

作者信息 +

Screening of differential metabolites in the urine of obese boys

LIU Wei^1*, DONG Jie^2*, GAO Chaonan¹, QI Qianjin¹, KONG Yawei¹, MENG Xin¹, PENG Xiaoxia³, YAN Yinkun¹

Author information +

文章历史 +

摘要

目的探究肥胖男童与体重正常男童之间尿液代谢物特征的差异,为肥胖的发病机制提供初步线索。方法采用病例对照研究设计,于2021年9—10月在湖南长沙三所寄宿学校招募6~14岁儿童,按体重状态分为肥胖组和体重正常组,将两组儿童按年龄1∶1进行匹配,最终纳入10名正常体重男童和10名肥胖男童。连续收集3d 24h尿样,混匀后取2mL尿样进行靶向检测。采用正交偏最小二乘法判别分析比较两组间代谢物的整体组成差异、t检验和差异倍数值相结合的方法筛选差异代谢物。结果肥胖组中吲哚-3-甲基乙酸酯、吲哚-3-丙酸、吲哚乙酸、胆酸、乙酰胆碱酸、石胆酸-3-硫酸盐、7-酮去氢胆酸、甲基半胱氨酸、脯氨酸和癸二酸的水平低于正常体重组,差异有统计学意义(Log₂FC≤-0.5,P<0.05)。结论肥胖男童与体重正常男童的尿液代谢物存在差异,可为肥胖的预防和控制提供新线索。

Abstract

Objective To investigate the differences in urinary metabolite profiles between obese and normal-weight boys, in order to provide preliminary clues for understanding the pathogenesis of obesity. Methods A case-control study design was employed. From September to October 2021, children aged 6 - 14 years were recruited from three boarding schools in Changsha, Hunan. Participants were divided into obese and normal-weight groups based on their weight status. Ten normal-weight boys and ten obese boys were matched by age(1∶1 ratio).24-hour urine samples were collected continuously for three days, mixed, and 2mL of urine was taken for targeted detection. Orthogonal partial least squares discriminant analysis(OPLS-DA) was used to compare the overall differences in metabolite composition between the two groups, and a combination of t-test and fold change(FC) values was employed to screen for differential metabolites. Result The levels of Indole-3-methyl acetate, Indole-3-propionic acid, Indoleacetic acid, Cholic acid, Glycocholic acid, Sulfated lithocholic acid, 7-dehydrocholic acid, Methylcysteine, Proline, and Sebacic acid were significantly lower in the obese group compared to the normal-weight group(Log₂FC≤-0.5, P <0.05). Conclusion Differences in urine metabolites exist between obese and normal-weight male children, which may provide new clues for the prevention and control of obesity.

导出引用

刘韦, 董洁, 高超男, 齐千瑾, 孔亚伟, 孟鑫, 彭晓霞, 闫银坤. 肥胖男童尿液差异代谢物的筛选[J]. 中国儿童保健杂志. 2025, 33(2): 180-184 https://doi.org/10.11852/zgetbjzz2024-0654

LIU Wei, DONG Jie, GAO Chaonan, QI Qianjin, KONG Yawei, MENG Xin, PENG Xiaoxia, YAN Yinkun. Screening of differential metabolites in the urine of obese boys[J]. Chinese Journal of Child Health Care. 2025, 33(2): 180-184 https://doi.org/10.11852/zgetbjzz2024-0654

中图分类号： R723.14

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