过敏原组分检测在儿童食物过敏诊断中的应用价值

doi:10.11852/zgetbjzz2022-1524

中国儿童保健杂志 ›› 2023, Vol. 31 ›› Issue (10): 1085-1090.DOI: 10.11852/zgetbjzz2022-1524

过敏原组分检测在儿童食物过敏诊断中的应用价值

卜华琴^1*, 宋瑜欣^2*, 周小勤^3*, 李在玲^4*, 谭琦^5*, 姚海丽¹, 孙晋波⁴, 于佳², 于晓华², 黄兆选³, 舒俊华³, 王华⁵, 孙金峤¹, 王晓川¹

1.国家儿童医学中心,复旦大学附属儿科医院临床免疫科,上海 201102;
2.哈尔滨市儿童医院变态反应科,黑龙江哈尔滨 150010;
3.湖北省妇幼保健院儿内科,湖北武汉 430064;
4北京大学第三医院儿科,北京 100191;
5.重庆医科大学附属儿童医院皮肤科,国家儿童健康与疾病临床医学研究中心,儿童发育疾病研究教育部重点实验室,重庆 400015

收稿日期:2022-12-20 修回日期:2023-05-04 发布日期:2023-10-23 出版日期:2023-10-10
通讯作者: 王晓川,E-mail:xchwang@shmu.edu.cn
作者简介:卜华琴(1997-),女,重庆人,硕士研究生在读,主要研究方向为儿童免疫与过敏;宋瑜欣(1962-),女,湖南人,本科学历,主要研究方向为儿童过敏性疾病;周小勤(1962-),女,江苏人,硕士学位,主要研究方向为儿童过敏性疾病;李在玲(1967-),女,四川人,博士学位,主要研究方向为儿童过敏性胃肠病;谭琦(1978-),男,湖南人,博士学位,主要研究方向为炎症性皮肤病。^*为共同第一作者。
基金资助:
国家重点研发计划(2021YFC2701802):上海市卫生健康学科带头人(2022XD024);儿科医院登峰计划(EK112520180202)。

Application value of allergen component analysis in the diagnosis of food allergy in children

BU Huaqin^1*, SONG Yuxin^2*, ZHOU Xiaoqin^3*, LI Zailing^4*, TAN Qi^5*, YAO Haili¹, SUN Jinbo⁴, YU Jia², YU Xiaohua², HUANG Zhaoxuan³, SHU Junhua³, WANG Hua⁵, SUN Jinqiao¹, WANG Xiaochuan¹

1. Department of Clinical Immunology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai 201102, China;
2. Department of Allergy, Children's Hospital of Haerbin Medical University, Haerbin,Heilongjiang 150010,China;
3. Department of Pediatrics, Maternal and Child Healthcare Hospital of Hubei Province,Wuhan,Hubei 430064,China;
4. Department of Pediatrics, Peking University Third Hospital, Beijing 100191,China;
5. Department of Dermatology, Children'sHospital of Chongqing 400015,China Medical University,National Clinical Research Center for Child Health and Disorders, Ministry of Education of Key Laboratory ofChild Developmental Disease Research,Chongqing 400015,China

Received:2022-12-20 Revised:2023-05-04 Online:2023-10-10 Published:2023-10-23
Contact: WANG Xiaochuan, E-mail: xchwang@shmu.edu.cn

摘要/Abstract

摘要： 目的比较过敏原特异性IgE(sIgE)和过敏原组分检测,探讨过敏原组分检测在食物过敏诊断中的应用价值。方法纳入2017年10月—2019年3月就诊于复旦大学附属儿科医院、哈尔滨市儿童医院、北京大学第三医院、湖北省妇幼保健院和重庆医科大学附属儿童医院的有过敏原及组分sIgE两种检测病史的儿童242例。分别采用ImmunoCAP系统和酶联免疫印迹法检测血清鸡蛋、牛奶、花生sIgE和相应的过敏原组分。结果 1)242例IgE介导的食物过敏儿童中,鸡蛋过敏162例、牛奶过敏152例和花生过敏72例,50例健康儿童作为对照。鸡蛋过敏儿童中,卵白蛋白的sIgE检测阳性率最高,其次是伴清蛋白和卵类粘蛋白,分别为96.3%(156/162)、92.6%(150/162)和83.9%(136/162);牛奶过敏儿童中,α-乳白蛋白的sIgE检测阳性率最高,其次是β-乳球蛋白、酪蛋白和牛血清白蛋白,分别为82.9%(126/152)、81.6%(124/152)、80.3%(122/152)和75.0%(114/152);花生各过敏原组分在花生过敏儿童中的sIgE检测阳性占比0%~13.8%。70%以上鸡蛋、牛奶过敏儿童同时对3种及以上过敏原组分致敏;鸡蛋过敏儿童的过敏原sIgE水平越高,致敏的过敏原组分种类越多(r=0.212,P=0.002)。2)鸡蛋、牛奶和花生过敏原组分检测的灵敏度分别为0.975、0.993和0.208,特异度分别为0.660、0.920和0.980,阳性预测值分别为0.903、0.974和0.938,阴性预测值分别为0.892、0.979和0.462,受试者工作特征曲线下面积及95%CI分别为0.818(95%CI:0.735~0.900)、0.957(95%CI:0.912~1.000)和0.594(95%CI:0.494~0.694)。结论食物过敏原组分检测可与过敏原sIgE检测同时作为诊断IgE介导的食物过敏的辅助检测手段,明确其致敏蛋白组分,更好地指导临床个体化管理食物过敏儿童。

关键词: 食物过敏, 过敏原组分检测, 过敏原特异性IgE, 儿童

Abstract: Objective To compare allergen specific immunoglobulin E (sIgE) with allergen component test in diagnosing food allergy, in order to explore the application value of allergen component test in diagnosing food allergy. Methods Children with history of allergen sIgE and allergen component test were enrolled in this study, from Children's Hospital of Fudan University, Harbin Children's Hospital, Peking University Third Hospital, Hubei Maternal and Child Health Hospital and Children's Hospital of Chongqing Medical University from October 2017 to March 2019. ImmunoCAP system and enzyme-linked immunoblotting were used to detect eggs, milk, peanuts sIgE and corresponding allergen components respectively. Results 1) Among 242 children with food allergy, 162 had egg allergies, 152 had milk allergies and 72 had peanut allergies, with 50 healthy children as controls. Among children with egg allergy, the highest rate of positive sIgE testing was for ovalbumin (96.3%,156/162), followed by ovotransferrin(92.6%,150/162) and ovomucoid(83.9%, 136/162). Among children with milk allergy, the highest rate of positive sIgE testing was for α-lactalbumin (82.9%, 126/152), followed by β-lactoglobulin (81.6%, 124/152), casein (80.3%, 122/152) and bovine serum albumin (75.0%, 114/152). The sIgE positivity for peanut allergen components in children with peanut allergy ranged from 0% to 13.8%. More than 70% of egg or milk allergies were sensitized to three or more allergen components concomitantly. The higher the level of allergen sIgE in children with egg allergy, the greater the variety of allergen components that cause allergy (r=0.212, P=0.002). 2) The sensitivity of the egg, milk and peanut allergen component test for diagnosing food allergy were 0.975, 0.993 and 0.208, respectively, with specificities of 0.660, 0.920 and 0.980, positive predictive values of 0.903, 0.974 and 0.938 and negative predictive values of 0.892, 0.979 and 0.462, respectively, and the area under the receiver operative characteristic curve were 0.818 (95%CI: 0.735 - 0.900), 0.957 (95%CI: 0.912 - 1.000) and 0.594 (95%CI: 0.494 - 0.694), respectively. Conclusion Food allergen component testing can be used in conjunction with allergen sIgE testing as an adjunct to the diagnosis of IgE-mediated food allergy, to identify the allergenic protein fraction and to better guide the individual clinical management of children with food allergy.

Key words: food allergy, allergen component testing, allergen specific IgE, children

中图分类号:

卜华琴, 宋瑜欣, 周小勤, 李在玲, 谭琦, 姚海丽, 孙晋波, 于佳, 于晓华, 黄兆选, 舒俊华, 王华, 孙金峤, 王晓川. 过敏原组分检测在儿童食物过敏诊断中的应用价值[J]. 中国儿童保健杂志, 2023, 31(10): 1085-1090.

BU Huaqin, SONG Yuxin, ZHOU Xiaoqin, LI Zailing, TAN Qi, YAO Haili, SUN Jinbo, YU Jia, YU Xiaohua, HUANG Zhaoxuan, SHU Junhua, WANG Hua, SUN Jinqiao, WANG Xiaochuan. Application value of allergen component analysis in the diagnosis of food allergy in children[J]. Chinese Journal of Child Health Care, 2023, 31(10): 1085-1090.

参考文献

[1] Sicherer SH, Sampson HA. Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management[J]. J Allergy Clin Immunol, 2018, 141(1): 41-58.
[2] Prescott SL, Pawankar R, Allen KJ, et al. A global survey of changing patterns of food allergy burden in children[J]. World Allergy Organ J, 2013, 6(1): 21-32.
[3] Hu Y, Chen J, Li HQ. Comparison of food allergy prevalence among Chinese infants in Chongqing, 2009 versus 1999[J]. Pediatr Int, 2010, 52(5): 820-824.
[4] Chen J, Hu Y, Allen KJ, et al. The prevalence of food allergy in infants in Chongqing, China[J]. Pediatr Allergy Immunol, 2011, 22(4): 356-360.
[5] 赵京. 中国儿童食物过敏现况[J]. 中华临床免疫和变态反应杂志, 2019, 13(4): 271-275.
Zhao J. Current situation of food allergy among children in China[J].Chinese Journal of Allergy & Clinical Immunology, 2019, 13(4): 271-275. (in Chinese)
[6] Dona DW, Suphioglu C. Egg allergy: Diagnosis and immunotherapy[J]. Int J Mol Sci, 2020, 21(14): 5010-5044.
[7] Knyziak-Mędrzycka I, Majsiak E, Cukrowska B. Allergic march in children: The significance of precision allergy molecular diagnosis (PAMD@) in predicting atopy development and planning allergen-specific immunotherapy[J]. Nutrients, 2023, 15(4): 978-988.
[8] 中华儿科杂志编辑委员会, 中华医学会儿科学分会. 儿童过敏性疾病诊断及治疗专家共识[J]. 中华儿科杂志, 2019, 57(3): 164-171.
Editorial Board of Chinese Journal of Pediatrics, Pediatrics Branch of Chinese Medical Association. Consensus on diagnosis and management of allergic disease in children[J]. Chin J Pediatr, 2019, 57(3): 164-171. (in Chinese)
[9] 王晓阳, 邵明军, 王宇璠, 等. 儿童IgE介导牛奶蛋白过敏的临床特征[J]. 中华儿科杂志 2022, 60(5): 447-451.
Wang XY, Shao MJ, Wang YF, et al. Clinical features of IgE-mediated cow's milk protein allergy in children[J]. Chin J Pediatr, 2022, 60(5): 447-451. (in Chinese)
[10] Palosuo K, Kukkonen AK, Pelkonen AS, et al. Gal d 1-specific IgE predicts allergy to heated egg in finnish children[J]. Pediatr Allergy Immunol, 2018, 29(6): 637-643.
[11] Dang TD, Peters RL, Koplin JJ, et al. Egg allergen specific IgE diversity predicts resolution of egg allergy in the population cohort HealthNuts[J]. Allergy, 2019, 74(2): 318-326.
[12] Ayats-Vidal R, Valdesoiro-Navarrete L, García-González M, et al. Predictors of a positive oral food challenge to cow's milk in children sensitized to cow's milk[J]. Allergol Immunopathol (Madr), 2020, 48(6): 568-575.
[13] Gunaydin NC, Severcan EU, Akarcan SE, et al. Effects of cow's milk components, goat's milk and sheep's milk sensitivities on clinical findings, and tolerance development in cow's milk allergy[J]. Sisli Etfal Hastan Tip Bul, 2021, 55(3): 391-397.
[14] D′auria E, Salvatore S, Pozzi E, et al. Cow's milk allergy: Immunomodulation by dietary intervention[J]. Nutrients, 2019, 11(6): 1399-1419.
[15] 张春梅, 赖荷, 陈蕴光, 等. 过敏原蛋白组分sIgE检测及分析[J]. 标记免疫分析与临床, 2017, 24(7): 725-729.
Zhang CM, Lai H, Chen YG, et al. Detection and ananlysis of allergic components specific IgE[J]. Labeled Immunoassays and Clinical Medicine, 2017, 24(7): 725-729. (in Chinese)
[16] 常雪娇, 李坤, 张英, 等. 晒干处理对花生过敏原蛋白潜在致敏性的影响[J]. 食品科学, 2018, 39(3): 49-54.
Chang XJ, Li K, Zhang Y, et al. Effect of sun-drying on potential allergenicity of peanut allergens[J]. Food Science, 2018, 39(3): 49-54. (in Chinese)
[17] Ott H,Baron JM,Heise R,et al.Clinical usefulness of microarray-based IgE detection in children with suspected food allergy[J].Allergy,2008,63(11):1521-1528.
[18] 唐川婷. 鸡蛋组分蛋白、半乳糖凝集素-3与过敏原IgE抗体联合检测在鸡蛋过敏患儿诊断中的价值[J]. 中国医药导报, 2018, 15(32): 88-91.
Tang CT. Diagnostic value of egg component protein, galactose agglutinin-3 and allergen IgE antibody applied in the diagnosis of children with egg allergy[J]. China Medical Herald, 2018, 15(32): 88-91. (in Chinese)

过敏原组分检测在儿童食物过敏诊断中的应用价值

Application value of allergen component analysis in the diagnosis of food allergy in children

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