支气管哮喘患儿血清IL-17和IL-36水平及临床意义

doi:10.11852/zgetbjzz2018-1507

中国儿童保健杂志 ›› 2019, Vol. 27 ›› Issue (6): 646-648.DOI: 10.11852/zgetbjzz2018-1507

支气管哮喘患儿血清IL-17和IL-36水平及临床意义

肖夏夏¹, 鲁珊珊¹, 申秋燕¹, 王晓静¹, 任丽芳¹, 孙中厚²

1 潍坊医学院,山东潍坊 261000;
2 潍坊市人民医院儿科,山东潍坊 261000

收稿日期:2018-10-24 发布日期:2019-06-10 出版日期:2019-06-10
通讯作者: 孙中厚,E-mail:szh65@sina.com
作者简介:肖夏夏(1991-),女,山东人,在读硕士研究生,主要研究方向为小儿呼吸与危重病。
基金资助:
山东省自然科学基金(ZR2010HL028)

Clinical significance and the levels of serum IL-17 and IL-36 in children with bronchial asthma

XIAO Xia-xia¹, LU Shan-shan¹, SHEN Qiu-yan¹, WANG Xiao-jing¹, REN Li-fang¹, SUN Zhong-hou²

1 Weifang Medical University, Weifang, Shandong 261000,China;
2 Pediatrics Department, Weifang People′s Hospital, Weifang, Shandong 261000, China

Received:2018-10-24 Online:2019-06-10 Published:2019-06-10
Contact: SUN Zhong-hou, E-mail:szh65@sina.com

摘要/Abstract

摘要： 目的探讨支气管哮喘患儿血清白细胞介素-17(IL-17)、白细胞介素-36(IL-36)水平和嗜酸性粒细胞(EOS)计数及临床意义。方法选取2017年7月-2018年7月在潍坊市人民医院小儿内科门诊就诊及病房住院哮喘儿童65名,其中哮喘急性发作期组儿童30名,缓解期组儿童35名,健康儿童25名作为对照组,采用酶联免疫吸附试验(ELISA)检测各组血清IL-17、IL-36水平,采用血细胞分析仪常规方法检测外周血EOS计数,并进行统计学分析。结果 1)急性发作期组和缓解期组IL-17水平分别为(45.73±8.86)pg/ml和(21.06±5.51)pg/ml,均显著高于对照组(7.29±4.29)pg/ml(P<0.01);且急性发作期组IL-17水平明显高于缓解期组(P<0.01)。2)急性发作期组和缓解期组IL-36水平分别为(53.76±10.60) pg/ml和(24.45±6.59) pg/ml,均显著高于对照组(8.46±4.86) pg/ml(P<0.01);且急性发作期组IL-36水平高于缓解期组,差异有统计学意义(P<0.01)。3)在哮喘患儿中,IL-17和IL-36水平呈正相关(P<0.01);且IL-17和IL-36水平分别与EOS计数呈正相关(P<0.01)。结论 IL-17和IL-36参与儿童哮喘发病过程,其血清水平可作为儿童哮喘诊断和病情评估较好的生物学指标。

关键词: 支气管哮喘, 白介素-17, 白介素-36, 嗜酸性粒细胞, 儿童

Abstract: Objective To analyze serum levels of interleukin-17(IL-17), interleukin-36 (IL-36) and eosinophil (EOS) count in children with bronchial asthma, and to discuss the clinical significance. Methods Totally 65 children with asthma were enrolled from July 2017 to July 2018 in Weifang People′s Hospital, of whom 30 cases were in acute exacerbation, 35 cases were in remission, and 25 health children were as control group. The serum levels of IL-17 and IL-36 were detected by enzyme-linked immunosorbent assay (ELISA), the eosinophil count in peripheral blood was measured by hematology analyzer, and the statistical analysis was performed. Results 1) The serum levels of IL-17 in acute exacerbation group[(45.73±8.86)pg/ml] and remission group[(21.06±5.51)pg/ml] were significantly higher than that in control group[(7.29±4.29)pg/ml] (P<0.01), and serum levels of IL-17 in acute exacerbation group was significantly higher than that in remission group(P<0.01). 2) The serum levels of IL-36 in acute exacerbation group[(53.76±10.60)pg/ml ] and remission group [(24.45±6.59) pg/ml] were significantly higher than that in control group[(8.46±4.86) pg/ml] (P<0.01), and serum level of IL-36 in acute exacerbation group was significantly higher than those in remission group(P<0.01). 3) In asthma children, serum level of IL-17 were positively correlated with the level of IL-36(P<0.01), and significant correlation were noted between the serum levels of IL-17, IL-36 and EOS count (P<0.01). Conclusion IL-17 and IL-36 are all involved in the pathogenesis of serum asthma in children, and serum levels of IL-17 and IL-36 may become a good biomarker for the diagnosis and evaluation of severity of asthma children.

Key words: bronchial asthma, interleukin-17, interleukin-36, eosinophil, children

中图分类号:

R725.6

肖夏夏, 鲁珊珊, 申秋燕, 王晓静, 任丽芳, 孙中厚. 支气管哮喘患儿血清IL-17和IL-36水平及临床意义[J]. 中国儿童保健杂志, 2019, 27(6): 646-648.

XIAO Xia-xia, LU Shan-shan, SHEN Qiu-yan, WANG Xiao-jing, REN Li-fang, SUN Zhong-hou. Clinical significance and the levels of serum IL-17 and IL-36 in children with bronchial asthma[J]. journal1, 2019, 27(6): 646-648.

参考文献

[1] 全国儿科哮喘协作组等. 第三次中国城市儿童哮喘流行病学调查[J]. 中华儿科杂志, 2013, 51(10): 729-735.
[2] Shin TS, Lee BJ, Tae YM, et al. Role of inducible nitric oxide synthase on the development of virus-associated asthma exacerbation which is dependent on Th1 and Th17 cell responses[J].Exp Mol Med,2010, 42(10): 721-730.
[3] Gresnlgt MS, van de Veerdon KFL. Biology of IL-36 cytokines and their role in disease[J]. Semin Immunol, 2013, 25(6): 458-465.
[4] 中华医学会儿科分会呼吸学组. 儿童支气管哮喘诊断与防治指南(2016年版)[J]. 中华儿科杂志, 2016, 54(3): 167-180.
[5] Pridgeon C, Bugeon L, Donnelly L, et al. Regulation of IL-17 in chronic inflammation in the human lung[J]. Clinical Science, 2011, 120(12): 515-524.
[6] Irvin C, Zafar I, Good J, et al. Increased frequency of dual-positive TH2/TH17 cells in bronchoalveolar lavage fluid characterizes a population of patients with severe asthma[J]. J Allergy Clin Immunol, 2014, 134(5): 1175-1186.
[7] Hallstrand TS, Lai Y, Altemeier WA, et al. Regulation and function of epithelial secreted phospholipase A2 group X in asthma[J]. Am J Respir Crit Care Med, 2013, 188(1): 42-50.
[8] 沈红燕, 杨丽萍, 杨芳. 支气管哮喘儿童血清IL-17、IL-35水平与肺功能的相关性分析[J].中国妇幼保健, 2017, 32(20): 5002-5004.
[9] Dietrich D, Martin P, Flacher V, et al. Interleukin-36 potently stimulates human M2 macrophages,langerhans cells and keratinocytes to produce pro-inflammatory cytokines[J]. Cytokine, 2016, 84: 88-98.
[10] Ramadas RA, Ewart SL, Medoff BD, et al. Interleukin-1 family member 9 stimulates chemokine production and neutrophil influx in mouse lungs[J]. Am J Resp Cell Mol, 2011, 44(2): 134-145.
[11] Ramadas RA, Ewart SL, Iwakura Y, et al. IL-36α exerts proinflammatory effects in the lungs of mice[J]. PLoS One, 2012, 7(9): e45784-e45784.
[12] 赵艳景,鲍文华,李树民,等. 支气管哮喘患者呼出气一氧化氮、血清IL-36水平及其相关性[J]. 临床肺科杂志, 2018, 23(2): 312-314.
[13] Dunn E, Sims JE, Nicklin MJH, et al. Annotating genes with potential roles in the immune system: six new members of the IL-1 family[J]. Trends Immunol, 2001, 22(10): 533-536.
[14] 王静静, 宋昕阳, 钱友存. 白介素-17家族细胞因子的研究进展[J]. 生命科学, 2016, 28(2): 170-181.

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支气管哮喘患儿血清IL-17和IL-36水平及临床意义

Clinical significance and the levels of serum IL-17 and IL-36 in children with bronchial asthma

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