产前感染对新生大鼠肺结缔组织生长因子表达的影响

doi:10.11852/zgetbjzz2017-25-06-09

中国儿童保健杂志 ›› 2017, Vol. 25 ›› Issue (6): 572-575.DOI: 10.11852/zgetbjzz2017-25-06-09

产前感染对新生大鼠肺结缔组织生长因子表达的影响

朱星华^{1, 2}, 李娟¹

1 中国医科大学附属盛京医院新生儿科,辽宁沈阳 110004;
2 中国医科大学附属第四医院儿科,辽宁沈阳 110032

收稿日期:2016-12-05 发布日期:2017-06-10 出版日期:2017-06-10
通讯作者: 李娟,E-mail:lijuan@sj-hospital.org
作者简介:朱星华(1986-),女,辽宁人,住院医师,硕士学位,主要研究方向为新生儿疾病。
基金资助:
辽宁省自然基金资助课题(201202273)

Effects of antenatal infection on the expression of connective tissue growth factor in lungs of neonatal rats

ZHU Xing-hua^{1, 2}, LI Juan¹

1 Department of Pediatrics,Shengjing Hospital of China Medical University,Shenyang,Liaoning 110004,China;
2 Department of Pediatrics,the Fourth Affiliated Hospital of China Medical University,Shenyang,Liaoning 110032,China

Received:2016-12-05 Online:2017-06-10 Published:2017-06-10
Contact: LI Juan,E-mail:lijian@sj-hospital.org

摘要/Abstract

摘要： 目的动态观察产前炎症暴露生后大鼠肺结缔组织生长因子(CTGF)表达和肺泡结构变化,探讨CTGF对肺发育的影响机制。方法 Wistar孕大鼠随机分为实验组和对照组,分别于孕19 d和20 d腹腔注射脂多糖(LPS2.5 mg/kg)和等量生理盐水,足月自然分娩后,于日龄1、3、7、14 d两组分别取8只仔鼠,麻醉处死后取肺组织,左肺行HE染色,观察肺组织结构,测量肺泡数量、肺泡间隔厚度、肺泡占组织面积比例,右肺采用Real time PCR和Western blot方法测定肺组织中CTGF mRNA及蛋白表达水平。结果 1)随日龄增加,两组大鼠肺泡数量、肺泡占组织面积比逐渐增加,肺泡间隔变薄。生后1 d、3 d、7 d及14 d,实验组肺泡数量明显少于对照组(P<0.05或<0.01);生后1 d、3 d和7 d,实验组肺泡占组织面积比明显大于对照组(P<0.01);生后1 d及3 d,实验组肺泡间隔厚度明显小于对照组(P=0.000)。2)生后1 d、3 d和7 d实验组CTGFmRNA表达明显高于对照组(P<0.05或<0.01)。3)生后1、3、7、 14 d实验组CTGF蛋白表达均明显强于对照组(P均<0.05)。结论产前感染导致生后大鼠肺CTGF表达增加与肺结构异常有关。

关键词: 产前感染, 肺发育, 结缔组织生长因子, 大鼠, 支气管肺发育不良

Abstract: Objective To explore the role for connective tissue growth factor(CTGF) in the lung development by dynamic observation of morphology and the expression of CTGF in lungs of rat pups exposed to antenatal inflammation. Methods Pregnant Wistar rats were randomly divided into experimental group and control group.Pregnant rats of both groups were intraperitoneal injected with either lipopolysaccharide ( LPS)2.5 mg/kg or the same volume of normal saline on embryonic day 19 and 20,respectively,and were allowed to term deliver.On postnatal days 1,3,7 and 14 (D1,D3,D7 and D14),eight pups of each group were killed by 10% chloral hydrate (1 ml/kg) and lungs were collected.Left lungs of each group were used to assess lung histological changes after hematoxylin and eosin(HE) staining by measuring the mean numbers of alveolar,the mean ratio of alveolar surface area to per tissue and the mean thickness of alveolar septum.Right lungs of each group were used to measure the expression of CTGF mRNA and protein by Real-time polymerase chain reaction (Real-time PCR) and Western blot. Results 1)With the increasing of postnatal days,the mean numbers of alveolar and the mean ratio of alveolar surface area to per tissue in both groups increased,the mean thickness of alveolar septum got thinner.On D1,D3,D7 and D14,the mean alveolar numbers of the experimental group were significantly less than those of the control group (P<0.05 or <0.01).On D1,D3 and D7,the mean ratios of alveolar surface area to per tissue were significantly larger than those of control group(P<0.01).On D1 and D3,the alveolar septum thickness was significantly thinner than that in control group(P=0.000).2)On D1,D3,andD7,the expression of CTGF mRNA of the experimental group were significantly higher than those of the control group(P<0.05 or <0.01).3)On D1,D3,D7 and D14,the expression of CTGF protein of the experimental group were significantly higher than those of the control group(P all<0.05). Conclusion Antenatal infection can up-regulate expression of CTGF,which may assiociate with disorders of lung development.

Key words: antenatal infection, lung development, connective tissue growth factor, rat, bronchopulmonary dysplasia

中图分类号:

R179

朱星华, 李娟. 产前感染对新生大鼠肺结缔组织生长因子表达的影响[J]. 中国儿童保健杂志, 2017, 25(6): 572-575.

ZHU Xing-hua, LI Juan. Effects of antenatal infection on the expression of connective tissue growth factor in lungs of neonatal rats[J]. journal1, 2017, 25(6): 572-575.

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产前感染对新生大鼠肺结缔组织生长因子表达的影响

Effects of antenatal infection on the expression of connective tissue growth factor in lungs of neonatal rats

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