Objective To compare the effects of three kinds of lipid emulsions to IL-6, and alveolar epithelial cell apoptosis of lipid emulsions (LPS)-induced rat lung of inflammatory. Methods One hundered young Sprague-Dawley rats were divided into control group, LPS group, intra group, Clino group and Omega group and then used normal saline or different fat emulsions for seven days.LPS was installed in order to establish the rat model of acute lung injury (ALI).Those rats were intratracheally instilled with normal saline or LPS 8 hours before sacrifice.Each lung tissue section's pathologic changes was observed, and apoptosis index(AI) was detected.Expression level of IL-6 mRNA was examined by RT-PCR, meanwhile the concentrations of IL-6 in BALF were measured by enzyme-linked immunospecific assay (ELISA). Results 1)The expressions of IL-6 mRNA in serum and the level of IL-6 in BALF of LPS groups and intra group were significantaly elevated than clino group and omega group (both P<0.005), but it was no significant difference(P＞0.005)when Clino group compared with Omega group.2)pulmonary histopathology:there were demonstrated significant inflammatory in light microscopy of HE-stained lungs tissues of rat, and they were showing inflammatory cells infiltrating around the bronchus and the vascular, even bleeding.3)apoptosis results:compared with LPS group and intra group, the AI of Clino group and Omega group significantly reduced(both P<0.005), which was no significant difference between each other(P＞0.005). Conclusion 20% clinoleic and 10% omegaven can relieve inflammation by downregulating the concentrations of IL-6, and reduce the alveolar epithelial cell apoptosis in rats with acute lung injury.
Key words
lipid emulsions /
acute lung injury /
IL-6
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