Objective To explore the role of thymus and activation regulated chemokine(TARC) in asthmatic rats and therapeutic effects of budesonide. Methods A total of 30 SD rats were randomly divided into normal group, asthmatic group, budesonide treated group, the rat model of asthma was established by the ovalbumin (OVA) provocation methods.The blood and bronchoalveolar lavage fluid (BALF) and lung tissue were collected 24 h after the last inhaling budesonide.The cells were counted, the concentrations of IL-4 and TARC in serum and BALF were measured by enzyme-linked immunosorbent assay (ELISA).The IL-4 and TARC protein in lung tissue were detected by immnohistochemical method. Results 1)The counts of eosinophiles, the IL-4 and TARC levels in serum and BALF of asthmatic groups were significantly higher than those in normal group(P<0.05), and the expressions of IL-4 and TARC protein in lung tissue of asthmatic groups were obviously higher than those in normal groups (P<0.05);They were significantly increased when inhaling budesonide (P<0.05).There were no significances between the control groups and the budesonide treated groups (P>0.05).2)Significant association was found between expression of TARC and IL-4 in the lung tissue of asthma groups and they presented for positive correlations (r=0.50, P<0.05). Conclusions TARC levels were increased in asthmatic rats.Inhaling budesonide can decrease the levels of TARC, TARC plays an important role in the pathogenesis of asthma.Downregulation of TARC expression may be the important mechanism of budesonide to treat asthma.
Key words
thymus and activation regulated chemokine /
asthma /
budesonide /
rats
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