Objective To explore the effects of early intervention on healthy personality development in infants and the evidence for optimized nurture and education. Methods A total of 88 newborns who were born from September to December 2007 in Zhongshan city and took periodic medical examinations in Boai Hospital of Zhongshan City were chosen and randomly assigned to early intervention group (46 cases) and control group (42 cases).Regular follow-up and general parenting guidance were performed for both groups.In addition to common health guidance, individualized instructions of healthy personality and training courses for parents were organized by well-trained professionals for early intervention group.The standard instruments used to assess the effect of intervention were Temperament Questionnaire (1~4 months old and 2.5~3 years old), Personality Tendency Scale for Children (PTSC) (2.5~3 years old), Achenbach Children Behavior Check list (CBCL) and Children′s Developmental Center of China (CDCC). Results The mean scores of PTSC (t=4.276, P<0.001), CDCC (Mental Development Index, t=4.775, P<0.01; Psychomotive Development Index, t=2.559, P<0.05) and Temperament (χ²=17.636, P=0.001) in the intervention group were significantly better than those in control group. Conclusion Early intervention can significantly promote the formation of a healthy personality of early childhood; it is a golden stage to cultivate infants' healthy personality in 0～3 years old.