Objective To examine the effect of granulocyte-colony stimulating factor (G-CSF) on survival of implanted human neural stem cells (hNSCs) and the differentiation into oligodendrocytes in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD rats, born within a week, were divided into two groups randomly:the single transplantation group (Group A, n=16) and the G-CSF + transplantation group (Group B, n=16), in which G-CSF[(50 μg/kg·d)]was administered 1 hour after performing operation subcutaneously once a day for five days in a row.In both group animals received implantation of hNSCs into the left cerebral ventricles at the second day after the operation.TUNEL staining was used to observe the apoptosis of the implanted cells in the rat brains 3 days post transplantation.While immunofluorescence was used to detect the survival and oligodendroglial differentiation 1 week and 2 weeks after the transplantation. Results Significantly decreased number of apoptosis cells was observed in group B compared to group A in 3 days after hNSCs transplantation (t=68.36, P<0.01).The grafted cells detected by DAPI survived, and generally distributed among cortex, hippocampus, striatum, and other areas.The statistical analysis revealed that DAPI positive cells were more in rats of group B than those of group A (t=14.07 and 2.5 respectively, P<0.05).Differentiated oligodendrocytes of implanted cells could scarcely be seen in either group. Conclusion The survival of implanted cells is increased and apoptosis is reduced with G-CSF treatment, which has no effect on the differentiation of implanted hNSCs into oligodendrocytes.
Key words
hypoxic-ischemic brain damage /
granulocyte-colony stimulating factor /
neural stem cells transplantation /
oligodendrocyte /
neonatal rats
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