Objective To realize working mother's breastfeeding practice, and analyse the related factors. Method 368 working mothers who had a child aged 6~24 months were investigated. Results The breastfeeding rate in 6 months was 72.8%, and the pure breastfeeding rate was 25.8%.The breastfeeding duration lasted (8.45±0.33) months, and the pure breastfeeding duration lasted (3.89±0.11) months.The main reasons for weaning were little milk and work stress.Cox regression analysis was used to analyze the breastfeeding duration influencing factors.Results showed longer breastfeeding duration mothers had characters as below:whose spouses having higher education, recurrent population, knowing "increasing suck times may increasing milk", knowing "breastfeeding should last 2 years", deciding to breastfeeding, having confidence on breastfeeding, maternity leave more than 6 months, having no or little work stress, and having the milking place in the working place. Conclusions The situation of working mother's breastfeeding practice is severe.Adjusting maternity leave and lactation leave, setting the nursey room in the working place, and helping for reduce work stress may prolong working mothers' breastfeeding duration.