Effect of erythropoietin on the expression of glial fibrillary acidic protein and S-100β induced brain injury in premature newborn rats intrauterine infection

SHANG Yun; MA Hui-min; GUO Xi-xia; SHI Ji-peng; WEN Zheng-fang; TANG Cheng-he; LI Shu-jun

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Chinese Journal of Child Health Care ›› 2014, Vol. 22 ›› Issue (10) : 1047-1050.

Effect of erythropoietin on the expression of glial fibrillary acidic protein and S-100β induced brain injury in premature newborn rats intrauterine infection

SHANG Yun¹,MA Hui-min²,GUO Xi-xia³,SHI Ji-peng¹,WEN Zheng-fang⁴,TANG Cheng-he¹,LI Shu-jun⁵

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Abstract

Objective To investigate the effects of recombinant erythropoietin(rhEPO) on expression of glial fibrillary acidic protein (EFAP) and S-100β in premature rat with intrauterine infection. Methods A total of 45 gestation 18 day pregnant rats were randomly divided into three groups,control group,received an intraperitoneal injection of normal saline,infection groups and intervention group were intraperitoneal injected with 0.45 mg/(kg·d) LPS continuting 2 days and the new rats in the intervention group were intraperitoneal injected with 5 000 U/(kg·d) rhEPO,continuting 3 days.Twenty-four hours after injection,part pregnant rats of each group were sacrificed.The pathological change of the placenta after HE staining was observed under light microscope.The glial fibrillary acidic protein (GFAP) and S-100β of the new 6 days rats' brains were examined by immunohistochemistry and enzyme linked immunosorbent assay(ELISA). Results Obvious pathological changes were observed in the placenta in the infection group and the intervention group.The GFAP on postnatal were 115.27±3.651 in control group,105.68±3.189 in infection group and 113.92±3.967 in intervention group and S-100β were (0.32±0.05 in control group 10.63±0.04 in infection group and 0.33±0.06 in intervention group.The GFAP and S-100β of infection group were significantly higher than those of control and intervention groups. Conclusions Intrauterine infection can increase the expression of GFAP and S-100βin the premature rats' cerebrum tisssue,rhEPO can decrease the expression of GFAP and S-100βand protect nervous system in neonatal rats with intrauterine infection made by LPS injection through abdominal cavity.

Key words

recombinant erythropoirth / newborn premature rat / the glial fibrillary acidic protein / S-100β

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SHANG Yun,MA Hui-min,GUO Xi-xia,SHI Ji-peng,WEN Zheng-fang,TANG Cheng-he,LI Shu-jun. Effect of erythropoietin on the expression of glial fibrillary acidic protein and S-100β induced brain injury in premature newborn rats intrauterine infection[J]. Chinese Journal of Child Health Care. 2014, 22(10): 1047-1050

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