Study on protective effect and its mechanism of pretreatment of  Caspase-3 gene inhibiter  on hypoxic-ischemic brain damage in the neonatal rats.

CHEN Hui-jun; YANG Yang.

PDF(436 KB)

Chinese Journal of Child Health Care ›› 2014, Vol. 22 ›› Issue (1) : 38-40.

Study on protective effect and its mechanism of pretreatment of Caspase-3 gene inhibiter on hypoxic-ischemic brain damage in the neonatal rats.

CHEN Hui-jun¹, YANG Yang².

Author information +

History +

Abstract

Objective To study the effect of hypoxia ischemia brain damage (HIBD) of neonatal rats with cerebral cortex apoptosis by gene inhibiter so as to explore the possible mechanism. Methods Model of HIBD was built in neonatal rats, 60 Wistar rats were randomly divided in:control group;HIBD group;obstruct group(HIBD+z-VAD-fmk).Adopting HE dyeing, three animal apoptosis index (AI) in brain and Caspase-3mRNA were detected by TUNEL and RT-PCR. Results Express of Caspase-3mRNA nanomolar in control group was significant heighten in HIBD group(P＜0.01).There was significantly lower express between obstruct group and HIBD group(P＜0.05).AI value changed with Caspase-3mRNA.They had significance positive correlation (r=0.628, P<0.05). Conclusion Express heigten of Caspase-3mRNA leads to histiocytic apoptosis in HIBD group, and lower express of Caspase-3mRNA by z-VAD-fmk, could protect brain of neonatal rats.

Key words

hypoxia ischemia damage / rat / apoptosis / Caspase-3

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CHEN Hui-jun, YANG Yang.. Study on protective effect and its mechanism of pretreatment of Caspase-3 gene inhibiter on hypoxic-ischemic brain damage in the neonatal rats.[J]. Chinese Journal of Child Health Care. 2014, 22(1): 38-40

References

[1] Okamura T, Miura T, Takuma G, et al.Effector caspase inhibitors on myocardial infarct size and myocyte DNA fragmentation in the ischemia-reperfused ratheart[J].Cardiovasc Res, 2000, 45(3):642-650.
[2] 李荣堂, 杨廷桐, 刘玲玲.缺血和缺血再灌注后交界区心肌细胞Caspase-3mRNA的动态变化与药物影响的研究[J].中原医刊, 2005, 32(18):3-5.
[3] Rice JE, Vannucci RC, Brierley JB.The inflluence of immaturity on hypoxic-ischemic brain damage in the rat[J].Ann Neurol, 1981, 9(2):131-141.
[4] 陈惠军.GM1对新生儿缺氧缺血性脑病IL-1β, NSE, ICAM-1的影响[J].山东医药, 2011, 51(41):39-40.
[5] 张媛媛, 李斐, 金星明, 等.生命早期前爪感觉和精细动作剥夺对大鼠海马Schaffer-CA1通路突触可塑性的影响[J].中国儿童保健杂志, 2010, 18(1):45-47.
[6] Abe T, Kunz A, Shimamura M, et al.The neuroprotective effect of pros-taglandin E2 EP1 receptor inhibition has a wideth erapeutic window, is sustained in time and is not sexually dimorphic[J].J Cereb Blood Flow Metab, 2009, 29(1):66-71.
[7] Xie NL, Zhang W, Li J, et al.Alpha-linolenic acid intake attenuates myocardial ischemia/reperfusioninjurythrough anti-inflammatory and anti-oxidative stress effects in diabetic but not normal rets[J].Aechives of Medical Research, 2011, 42(3):171-181.
[8] Chichkova NV, Shaw J, Galiullina RA, et al.Phytaspasw a relocalisable cell death promoting plant protease with caspase specificity[J].EMBO Journal, 2010, 29(6):1149-1161.
[9] Mami Y, Yayoi E, Toshii I, et al.Quantification of activated and total caspase-14 with newly developed ELISA systems in nirmal and atopic skin[J].J Dermatol Sci, 2011, 61(2):110-117.
[10] Sirbulescu RF, Zupanc CK.Inhibition of caspase-3 mediated apoptosis improves spinal cord repair a regeneration-competent vertebrate system[J].Neu Sci, 2010, 26(2):126-131.
[11] Van Empel VP, Bertrand AT, Hofstra L, et al.Myocyte apoptosis in heart failure[J].Cardiovasc Resc, 2005, 67(1):21-29.
[12] Wang Y, Qin NH.Molecular ang cellular mechanisms of excitotoxic neuronal death[J].Apoptosis, 2010, 7(5):798-806.
[13] Saitoh T, Nakajima T, Takahashi T, et al.Changes in cardiovascular funetion on treatmentof inhibitors of apoptotic signal transduction pathways in left ventricular remodeling after myocardial infarction[J].Cardiovasc Path, 2006, 15(3):130-138.
[14] Niizuma K, Yoshioks H, Chen H, et al.Mitochondrial and apoptotic neuronal death signaling pathways in cerebral ischemia[J].Biochim Biophy Acta, 2010, 1802(1):92-99.
[15] Matsuura S, Egi Y, Yuki S, et al.Mp-124 a novel poly (ADP-ri-bose) polymerase-1(PARP-1)inhibitor ameliorales ischemic brain damage in a non-human primate model[J].Brain Res, 2011, 1410(1):122-131.