Objective To investigate the effects of ω-3 polyunsaturated fatty acids (ω-3PUFAs) to LPS-induced rat lung(ALI) of inflammatory:TNF-α, IL-1 β and IL-6. Methods Sixty young SD rats were divided into control group, LPS group and omega group and then used normal saline or different fat emulsions for seven days.The rat model of ALI was established by instill LPS.Those groups were sacrificed at 8 hours after intratracheal introduction of LPS.Each lung tissue section's pathologic changes was observed, meanwhile the concentrations of TNF-α, IL-1 β and IL-6 in BALF were measured by enzyme-linked immunospecific assay (ELISA). Results 1)Pulmonary histopathology:ALI modle groups showed inflammatory cells infiltrating around the bronchus and the vascular, even bleeding and more apoptosis cells.Omega group was significantly lighter than LPS group (P<0.01).2)Compared with control group, the lung indexes and pathological scores of ALI model groups were significantly higher (P<0.05).3) The levels of TNF-α, IL-1β and IL-6 in BALF of LPS group were significantely elevated than omega group (both P＜0.05). Conclusion 10%Omegaven can relieve inflammation by downregulating the concentrations of TNF-α, IL-1β and IL-6 and reduce lung injury.
Key words
ω-3PUFAs /
acute lung injury /
TNF-α /
IL-1β /
IL-6
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