Cultivation, differentiation and identification in vitro of the neural stem cells from neonatal rat.

WANG Yu; YIN Xiao-juan.

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Chinese Journal of Child Health Care ›› 2014, Vol. 22 ›› Issue (1) : 31-34.

Cultivation, differentiation and identification in vitro of the neural stem cells from neonatal rat.

WANG Yu^{1, 2}, YIN Xiao-juan^{1, 2}.

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Abstract

Objective To investigate the cultivation, identification and identification cultivation of the neural stem cells (NSCs) from neonatal rats. Methods Hippocampus was isolated from neonatal SD rats and NSCs were cultured in serum free medium.Nestin expression in NSCs was detected by immunohistochemical fluorescence.The proliferation of NSCs was assessed by both of 5-bromodeoxyuridine (5-BrdU) incorporation assay and double-marked immunofluorescence.The differentiation of NSCs was induced.To identify the differentiated NSCs, antibodies of neuron-specific enolase (NSE) and antibody of glial fibrillary acidic protein (GFAP) were employed respectively with immunohistochemical fluorescence staining. Results A mass of neurospheres were obtained.Those NSCs could differentiate into neurons and astrocytes. Conclusions The fetal rat NSCs are successfully obtained, which have the capacities of proliferation, self-renew and pluripotentiality.They could differentiate into neurons and astrocytes, lay the foundation for the basic and clinical research in vitro.

Key words

neonatal rat / neural stem cells / cell culture / immunohistochemistry

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WANG Yu, YIN Xiao-juan.. Cultivation, differentiation and identification in vitro of the neural stem cells from neonatal rat.[J]. Chinese Journal of Child Health Care. 2014, 22(1): 31-34

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