Objective To study the effects of sodium valproate(VPA )on homer1a and mGluR5 expression in Hippocampal tissue of epileptic rats. Methods SD rats were divided into normal control(NC) group,pentetrazole (PTZ) group and VPA group.PTZ was intraperitoneally injected once every 48 h.Fully kindled rats of VPA group were injected with VPA[15mg/(kg·d)],while the rats of PTZ group were injected with normal saline.After 30 minutes,seizures were induced with the same dose of PTZ.Realtime-PCR was performed to examine the mRNA expression of Homer1a and mGluR5,Western-blot was used to detect the protein expression of them.Results 1)The rats of NC group had no seizure activity;those of VPA group showed slighter seizure activity than PTZ group.2)The expression level of Homer1a increased from 8 h after the last seizure,and decreased to the normal level 24 h later;the level of both them in VPA group increased in 8 h and 24 h after the last seizure.3)The level of mGluR5 in 8 h after the last seizure had no signifitant difference compared to NC group.mGluR5 expression was upregulated in 24 h after seizure and was downregulated in VPA group. 【Conclution】 1)VPA can alleviate the degree of seizure.2)Homer1a can increase transiently in early phase of epileptogenesis,mGluR5 can be upregulated in recovery phase.3)VPA may affect the expression of mGluR5 by regulating Homer1a for anti-epileptogenesis.
Key words
epilepsy /
valproate /
Homer1a /
mGluR5
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