Objective To observe the influence of extrinsic estrogen on the reproductive system of prepubertal male rats. Methods A total of 45 rats were randomly divided into high dose group,low dose group and control group.E2B was administered through gastric tube in male rats with 15 000 μg/kg,15 μg/kg,0 μg/kg,dose respectively.One time every two days,after three weeks,normal breeding to ninth weeks,then put to death and draw materials,and measured corresponding index. Results Compared with control group,T concentration of low dose decreased(P<0.05),but FSH,LH and E2 concentration increased,the differences were statistically significant (P<0.01).Four kinds of hormone of high dose changed more apparent(P<0.01).In two experimental group,the differences of T,FSH,LH and E2 concentration had statistical significances (P<0.05).The testicular reproductive small tube and AGD of two experimental group were both abnormal changed,but changes in high dose group were more obvious than those in low group.Conclusion EES can change the normal serum sex hormone levels and reproductive small tube of preadolescence male rats.
Key words
extrinsic estrogen /
estradiol benzoate /
preadolescence /
rats /
reproductive system
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] 贺厚光,张炜,吴天麟,等.苯甲酸雌二醇诱导小鼠尿道下裂动物模型的建立[J].中华男科学杂志,2007,13(1):3-7.
[2] 张泉,周新华,张孝斌,等.苯甲酸雌二醇对大鼠睾丸组织发育的影响[J].中华男科学杂志,2005,11(7):490-493,498.
[3] Putz O,Schwartz CB,LeBlanc GA,et al.Neonatal low- and high-dose exposure to estradiol benzoate in the male rat:II.Effects on male puberty and the reproductive tract[J].biology of reproduction,2001,65:1506-1517.
[4] Adriani W,Seta DD,Fulgheri FD,et al.Altered profiles of spontaneous novelty seeking,impulsive behavior,and response to D-amphetamine in rats perinatally exposed to bisphenol A[J].Environ Health Perspect,2003,11(4):395-401.
[5] 孙敬方.动物实验方法学[M].北京:人民卫生出版社,2001:21-25.
[6] 李和程,陈琦,王子明,等.青春期前己烯雌酚暴露对大鼠性成熟后生精细胞凋亡的影响及其机制初探[J].中华男科学杂志,2006,12(9):814-818.
[7] O'Donnell L,Robertson KM,Jones ME,et al.Estrogen and sperma-togenesis[J].Endocr Rev,2001,22(3):289-318.
[8] Aksglaede L,Juul A,Leffers H,et al.The sensitivity of the child to sex steroids:possible impact of exogenous estrogens[J].Hum Reprod Update,2006,12(4):341-349.
[9] Sanchez-Criado JE,De-Las Mulas JM,Bellido C,et al.Biological role of pituitary estrogen receptors ERalpha and ERbeta on progesterone receptor expression and action and on gonadotropin and prolactin secretion in the rat[J].Neuroendocrinology,2004,79(5):247-258.
[10] 李健,位兰,薛帮群,等.环境雌激素的雄性生殖毒性作用及机制[J].畜牧与饲料科学,2011,32(3):81-82.
PDF(464 KB)
