Effects of intermittent hypoxia on cognition and the ultrastructure of hippocampal synapses in immature rats.
- CHEN Yan1,ZHAO Chun-ling1,YU Guang1,CHENG Ji-yan2.
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Abstract
Objective To study the progressive effects of chronic intermittent hypoxia(CIH) on cognition and the ultrastructure of hippocampal synapses in immature rats. Methods A total of Sixty-six healthy,male,21-day-old Sprague-Dawley immature rats,weighing (50±5) g,was randomly averagely divided into two groups:unhandled control(UC)group and CIH group.Every group was divided into three time points:2,4 and 6 weeks(n=11).CIH-handled immature rats were exposed to intermittent hypoxia in designed cabin 8 hours every day.The cognitive function was assessed by the Morris Water Maze(MWM).The ultrastructure of hippocampal synapses were observed by electron microscope. Results 1)MWM of place navigation test:the escape latency in CIH immature rats was significantly longer compared with that in UC rats(P< 0.05);2)MWM of spatial probe test:the percentage of time spent on crossing the target quadrant to the total swimming time in CIH group was significantly decreased compared with that in UC group(P<0.05).The number of times of crossing the platform in CIH group was significantly reduced compared with that in UC group(P<0.05);3)Changes in the ultrastructure of hippocampal synapses were obvious in CIH group as compared with the UC group. Conclusion Chronic intermittent hypoxia induced slowly progressive cognition impairments in immature rats,which maybe associated with the changes in the ultrastructure of hippocampal synapses exposed to CIH.
Key words
hypoxia / cognition / hippocampus / ultrastructure / synapses / immature rats
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References
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[15] Hoffmann H,Hunt P,Spear NE.Ontogenetic differences in the association of gustatory and tactile cues with lithium chloride and footshock[J].Behav Neural Biol,1990,53(3):441-450.
