Objective To establish the normal reference ranges of myocardial enzyme series of each age group for the healthy children in Songzi city by testing the healthy children's myocardial enzymes. Methods 957 healthy children's peripheral venous blood were collected serum AST,CK,CK-MB,LDH and α-HBDH levels were determined by kinetic rate method on Architect C-8000 Aerosef. Results The normal reference value of myocardial enzyme was reduced gradually with aging.The differences of serum AST,CK,CK-MB,LDH and α-HBDH levels were significant among the four groups(P＜0.05 or ＜0.01).The levels of myocardial enzymes of GroupⅠ(24~168 h,newborn group)were obviously higher than of Group Ⅱ(~3 years old,infants group),Group Ⅲ(~6 years old,preschool group)and Group Ⅳ(~12 years old,school-aged group).The myocardial enzyme values of children in all groups were significantly higher than the normal reference ranges of the adults control group(P＜0.05 or ＜0.01).The normal reference ranges of myocardial enzymes in all of the four age groups were as follows:GroupⅠ,AST 23~113 U/L;CK 194~1104 U/L;CK-MB 25~115 U/L;LDH 140~956 U/L and α-HBDH 92~754 U/L;GroupⅡ,AST 19~73 U/L;CK 37~237 U/L;CK-MB 7~55 U/L;LDH 176~414 U/L and α-HBDH 122~320 U/L;Group Ⅲ,LDH 165~343 U/L and α-HBDH 102~274 U/L;Group Ⅳ,AST 13~49 U/L;CK 20~186 U/L;CK-MB 5~39 U/L;LDH 126~286 U/L and α-HBDH 86~220 U/L. Conclusion The establishment of adaptive normal reference ranges of myocardial enzyme series for the local newborns,infants,preschool children and school-aged children would provide reliable indexes for clinical diagnosis and treatment.