Changes of matrix metalloproteinase 2 and matrix metalloproteinase 9 in meconium injured young rabbits treated by proportional assist ventilation.
- WU Rong1,ZHOU Hai-yan1,LI Na1,FENG Zhi-chun2.
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Abstract
Objective To observe the changes of matrix metalloproteinase 2(MMP-2) and matrix metalloproteinase 9(MMP-9) in severe meconium-injured young rabbits treated by PAV. Methods Thirty 20~30 days rabbits were randomly divided into four groups:implement proportional assist ventilation after poured into the meconium(PAV group),implement synchronized intermittent mandatory ventilation after meconium instillation(SIMV group),no ventilation after meconium instillation(MAS group) and the saline irrigation with no ventilation group(control group).The rabbits were sacrificed after 8 hours,lungs and trachea were excised.Levels of MMP-2 and MMP-9 in lung homogenate and bronchoalveolar lavage fluid(BALF) were measured by enzyme-link immunosorbent assay(ELISA). Results 1) There were statistically significant differences among the lung tissue MMP-2(F=72.575,P=0.000) and MMP-9 levels of all groups.Between the lung tissue MMP-2,MMP-9 (F=48.374,P=0.000) levels of every group,the differences were significant(all P<0.05).The value of SIMV group was highest,the value of control group was lowest.2) There were statistically significant differences among the BALF MMP-2(F=116.779,P=0.000) and MMP-9(F=59.095,P=0.000)levels of all groups.Between the BALF MMP-2 and MMP-9 levels of every group,all the differences were significant(all of the P<0.05).The value of SIMV group was hightest,the value of control group was lowest.3) There were statistically significant differences in PIP at 2 h(F=46.095,P=0.000)、4 h(F=50.757,P=0.000)、6 h(F=56.127,P=0.000) and 8 h(F=73.684,P=0.000) after ventilation between PAV group and SIMV group,the value of SIMV group was higher than PAV. Conclusions In case to maintain normal oxygen saturation and blood gas,the values of MMP-2,MMP-9 and PIP in SIMV model were higher than PAV model.It suggest that when the MAS surported with mechanical ventilation,the priority is given to PAV model.
Key words
matrix metalloproteinase / proportional assist ventilation / meconium aspiration syndrome / lung injury
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References
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[2] Kacmarek RM.Proportional assist ventilation and neurally adjusted ventilatory assist[J].Respir Care,2011,56:140-152.
[3] 李丛锋,朱光发.基质金属蛋白酶2、基质金属蛋白酶9与急性肺损伤关系的研究进展[J].国际呼吸杂志,2011,31(14):1097-1101.
[4] 周海燕,武荣.新生兔重度胎粪吸入模型的制备[J].安徽医学,2010,31(9):967-969.
[5] Lagente V,Manoury B,Nenan S,et al.Role of matrix metalloproteinases in the development of airway inflammation and remodeling[J].Braz J Med Biol Res,2005,38(10):1521-1530.
[6] 张向峰,朱光发,刘双,等.基质金属蛋白酶-2/9及其组织抑制剂比例失衡在高氧所致急性肺损伤中的作用[J].中国危重病急救医学,2008,20(10):597-600.
[7] Pirrone F,Pastore C,Mazzola S,et al.In vivo study of the behaviour of matrix metalloproteinases(MMP-2/MMP-9) in mechanical,hypoxic and septic-induced acute lung injury[J].Vet Res Commun,2009,33(S1):12l-124.
[8] Haseneen NA,Vaday GG,Zucker S,et al.Mechanical stretch induces MMP-2 release and activation in lung endothelium:role of EMMPRIN[J].Am J Physiol Lung Cell Mol Physiol,2003,284:541-547.
[9] 武荣,周海燕,李娜,等.比例辅助通气治疗幼兔胎粪吸入的肺组织病理观察[J/CD].中华临床医师杂志:电子版,2011,5(19):5690-5693.
[10] 武荣,周海燕,李娜,等.比例辅助通气对重症胎粪吸入幼兔肺组织肿瘤坏死因子-α和白细胞介素-8水平的影响[J].中国新生儿科杂志,2012,27(3):201-204.
[11] Kamat M,Wu SY,Yeh TF.Meconium Aspiration Syndrome-pathogenesis and current management[J].Neonatology Today,2009,4:1-8.
[12] Schulze A,Bancalari E.Proportional assist ventilation in infants[J].Clin Perinatol,2001,28(3):561-578.
[13] 周晓光.新生儿常见疾病的机械通气策略[J].实用儿科临床杂志,2009,24(6):401-404.
[14] 李娜,周海燕,武荣.不同通气模式在新生兔重症胎粪吸入综合症的应用比较[J].安徽医学,2011,32(1):23-25.
[15] 武荣,李娜,周海燕.比例辅助通气与持续正压通气在幼兔胎粪吸入模型上的应用比较[J].重庆医学,2012,41(10):968-969.
