Objective To investigate the conditions of physical fitness of 3~6-year-old children in Kunming,and evaluate the physical status. Methods A cross-sectional investigation was conducted among 3 kindergartens in urban area of Kunming city by random cluster sampling.767 children were investigated by using "The Test Standard of Physical Fitness for Children in 3~6 years old".Collecting data were further to analyze by different statistics methods. Results The general status of physical fitness evaluation was not quite satisfied and the failed rate was 24.5%;The children's physical quality development was not balanced,the pass rates of the sub-items in the speed and sensitivity ability were higher,but the pass rates of the sub-items in the strength,endurance and balance ability were lower.The overweight/obese children grades in the all 8 sub-items were lower than normal children,especially in Continued jumping with both feet and balance beam. Conclusions Taking exercise is very important for the health of 3~6 years old children.The parents and health care workers of kindergartens should pay much attention to it.The children physical fitness evaluation should be an effective method in future childhood obesity intervention.